|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Clone||DAK Calret 1|
|Immunogen||Recombinant full length human calretinin protein cloned from Mero-41 cells|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:20|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12539 targets Calretinin in IHC (P) applications and shows reactivity with Human samples.
The MA5-12539 immunogen is recombinant full length human calretinin protein cloned from Mero-41 cells.
Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High adhesion of tumor cells to mesothelial monolayers derived from peritoneal wash of disseminated gastrointestinal cancers.
MA5-12539 was used in immunocytochemistry to study the role of the mesothelial layer in tumor cell adhesion using layers of mesothelial cells derived from gastrointestinal cancers
|Ranieri D,Raffa S,Parente A,Rossi Del Monte S,Ziparo V,Torrisi MR||PloS one (8:null)||2013|
Biphasic malignant mesothelioma cases with osseous differentiation and long survival: a review of the literature.
MA5-12539 was used in immunohistochemistry to report two clinical cases of biphasic malignant mesothelioma with osseous differentiation
|Demirag F,Unsal E,Tastepe I||Lung cancer (Amsterdam, Netherlands) (57:233)||2007|