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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full-length mouse calretinin protein|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 5 publications below|
This antibody is predicted to react with bovine, chicken and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is mesothelioma.
Recognizes a protein of 31.5kDa, identified as Calretinin. Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural mitif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gonadoblastoma: Case report of two young patients with isochromosome 12p found in the dysgerminoma overgrowth component in one case.
MA1-39562 was used in immunohistochemistry to report on two cases of gonadoblastoma
|Changchien YC,Haltrich I,Micsik T,Kiss E,Fónyad L,Papp G,Sápi Z||Pathology, research and practice (208:628)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA1-39562 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.
MA1-39562 was used in immunohistochemistry to investigate deciduoid features of malignant mesothelioma by histomorphological and immunohistochemical evaluation
|Ustun H,Astarci HM,Sungu N,Ozdemir A,Ekinci C||Diagnostic cytopathology (39:402)||2011|
Perivascular epithelioid tumor (PEComa) of the falciform/ broad ligament.
MA1-39562 was used in immunohistochemistry to report on a case of perivascular epithelioid tumor (PEComa) of the falciform/ broad ligament
|Ryś J,Karolewski K,Pudełek J,Kruczak A,Wasilewska A,Vogelgesang M,Kojs Z||Polish journal of pathology : official journal of the Polish Society of Pathologists (59:211)||2009|
Confusing cases: clear cell but not renal cell lesions in kidney.
MA1-39562 was used in immunohistochemistry to examine the diagnostic process for clear cell and renal cell lesions in kidney
|Baydar D,Aydin O||Pathology international (58:713)||2008|
29 kDa calbindin; CAL2; calbindin 2, (29kD, calretinin); calbindin D29K; calretinin; testicular secretory protein Li 8
CAB29; CAL2; CALB2; CR