|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full-length mouse calretinin protein|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is mesothelioma.
This antibody recognizes a protein of 31.5kDa, identified as Calretinin. Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Recurrent renal cell carcinoma manifesting as a large intrathoracic fibrotic mass: A case report.
MA5-16328 was used in immunohistochemistry - paraffin section to examine a case of recurrent renal cell carcinoma manifesting in the thorax as a fibrotic mass
|Kim JH,Jeong JH,Park SH,Jeong JS,Ryu YJ,Song SY||Oncology letters (11:3835)||2016|
|Not Applicable||Not Cited||
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
MA5-16328 was used in immunohistochemistry to study a northern New England hospital and increased utilization, verification, and clinical implications of immunocytochemistry
|Sauter JL,Ambaye AB,Mount SL||Diagnostic cytopathology (43:688)||2015|
Gonadoblastoma: Case report of two young patients with isochromosome 12p found in the dysgerminoma overgrowth component in one case.
MA5-16328 was used in immunohistochemistry to report on two cases of gonadoblastoma
|Changchien YC,Haltrich I,Micsik T,Kiss E,Fónyad L,Papp G,Sápi Z||Pathology, research and practice (208:628)||2012|
Leiomyomatosis peritonealis disseminata associated with endometriosis and multiple uterus-like mass: report of two cases.
MA5-16328 was used in immunohistochemistry to present two cases of leiomyomatosis peritonealis
|Carvalho FM,Carvalho JP,Pereira RM,Ceccato BP,Lacordia R,Baracat EC||Clinical medicine insights. Case reports (5:63)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA5-16328 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Perivascular epithelioid tumor (PEComa) of the falciform/ broad ligament.
MA5-16328 was used in immunohistochemistry to report on a case of perivascular epithelioid tumor (PEComa) of the falciform/ broad ligament
|Ry¿ J,Karolewski K,Pude¿ek J,Kruczak A,Wasilewska A,Vogelgesang M,Kojs Z||Polish journal of pathology : official journal of the Polish Society of Pathologists (59:211)||2009|