|Tested species reactivity||Human, Rat|
|Published species reactivity||Ferret, Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A fusion protein containing the first 99 amino acid residues from human CB1.|
|Storage buffer||PBS with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-743 detects cannabinoid receptor 1 (CB1) from human and rat tissues as well as transfected rat CB1.
PA1-743 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~52 kDa protein representing CB1 from rat brain homogenate. Immunocytochemical staining of CB1 in AtT20 cells transfected with the rat CB1 gene with PA1-743 yields a pattern consistent with plasma membrane staining.
PA1-743 antigen is a fusion protein containing the first 99 amino acid residues of human CB1.
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels.
CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neonatal Treatment with a Pegylated Leptin Antagonist Induces Sexually Dimorphic Effects on Neurones and Glial Cells, and on Markers of Synaptic Plasticity in the Developing Rat Hippocampal Formation.
PA1-743 was used in immunohistochemistry to study the effect of the neonatal leptin surge on hippocampal formation
|López-Gallardo M,Antón-Fernández A,Llorente R,Mela V,Llorente-Berzal A,Prada C,Viveros MP||Journal of neuroendocrinology (27:658)||2015|
Cannabinoid CB1 receptor is expressed in chromophobe renal cell carcinoma and renal oncocytoma.
PA1-743 was used in immunohistochemistry to study the expression of CB1 and CB2 cannabinoid receptor mRNA and protein in chromophobe renal cell carcinoma and renal oncocytoma
|Larrinaga G,Sanz B,Blanco L,Perez I,Candenas ML,Pinto FM,Irazusta A,Gil J,López JI||Clinical biochemistry (46:638)||2013|
Spinal cord injuries induce changes in CB1 cannabinoid receptor and C-C chemokine expression in brain areas underlying circuitry of chronic pain conditions.
PA1-743 was used in immunohistochemistry to study the effect of spinal cord injuries on cannabinoid receptor and chemokine expression in brain areas involved in pain perception
|Knerlich-Lukoschus F,Noack M,von der Ropp-Brenner B,Lucius R,Mehdorn HM,Held-Feindt J||Journal of neurotrauma (28:619)||2011|
CB1 and CB2 cannabinoid receptor expression during development and in epileptogenic developmental pathologies.
PA1-743 was used in immunohistochemistry to study the role of the CB1 and CB2 cannabinoid receptors in epileptogenic developmental pathologies
|Zurolo E,Iyer AM,Spliet WG,Van Rijen PC,Troost D,Gorter JA,Aronica E||Neuroscience (170:28)||2010|
Regulation of subthalamic neuron activity by endocannabinoids.
PA1-743 was used in immunohistochemistry to study the role of endocannabinoids in the regulation of subthalamic neuron activity
|Morera-Herreras T,Ruiz-Ortega JA,Taupignon A,Baufreton J,Manuel I,Rodriguez-Puertas R,Ugedo L||Synapse (New York, N.Y.) (64:682)||2010|
Involvement of the endocannabinoid system in periodontal healing.
PA1-743 was used in immunohistochemistry to study the role of endocannabinoid receptors in periodontal healing
|Kozono S,Matsuyama T,Biwasa KK,Kawahara K,Nakajima Y,Yoshimoto T,Yonamine Y,Kadomatsu H,Tancharoen S,Hashiguchi T,Noguchi K,Maruyama I||Biochemical and biophysical research communications (394:928)||2010|
Activated endocannabinoid system in coronary artery disease and antiinflammatory effects of cannabinoid 1 receptor blockade on macrophages.
PA1-743 was used in immunohistochemistry to study the possible role of the endocannabinoid system in patients with CAD and CB2 receptor blockade's antiinflammatory effect on human macrophages
|Sugamura K,Sugiyama S,Nozaki T,Matsuzawa Y,Izumiya Y,Miyata K,Nakayama M,Kaikita K,Obata T,Takeya M,Ogawa H||Circulation (119:28)||2009|
Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.
PA1-743 was used in immunohistochemistry to study the expression of cannabinoid CB2 receptors and fatty acid amide hydrolase in neuritic plaque-associated glia in Alzheimer disease brains.
|Benito C,Núñez E,Tolón RM,Carrier EJ,Rábano A,Hillard CJ,Romero J||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:11136)||2003|
Delta9-tetrahydrocannabinol selectively acts on CB1 receptors in specific regions of dorsal vagal complex to inhibit emesis in ferrets.
PA1-743 was used in immunohistochemistry to investigate the antiemetic effect of THC in the ferret
|Van Sickle MD,Oland LD,Mackie K,Davison JS,Sharkey KA||American journal of physiology. Gastrointestinal and liver physiology (285:G566)||2003|
Cannabinoids acting on CB1 receptors decrease contractile performance in human atrial muscle.
PA1-743 was used in immunohistochemistry to investigate the impact of cannabinoids on human atrial heart muscle
|Bonz A,Laser M,Küllmer S,Kniesch S,Babin-Ebell J,Popp V,Ertl G,Wagner JA||Journal of cardiovascular pharmacology (41:657)||2003|
A Role for the Cannabinoid 1 Receptor in Neuronal Differentiation of Adult Spinal Cord Progenitors in vitro is Revealed through Pharmacological Inhibition and Genetic Deletion.
PA1-743 was used in immunocytochemistry to use knock out technology and pharmacological inhibition to elucidate the role of the cannabinoid-1 receptor in the neuronal differentiation of adult spinal cord progenitors
|Sideris A,Bekker T,Chan WS,Montoya-Gacharna JV,Blanck TJ,Recio-Pinto E||Frontiers in neuroscience (6:null)||2012|
Effect of capacitation on the endocannabinoid system of mouse sperm.
PA1-743 was used in western blot to investigate the effect of capacitation on the spermic endocannabinoid system in mice
|Catanzaro G,Battista N,Rossi G,Di Tommaso M,Pucci M,Pirazzi V,Cecconi S,Maccarrone M||Molecular and cellular endocrinology (343:88)||2011|
Effects of chronic bhang (cannabis) administration on the reproductive system of male mice.
PA1-743 was used in western blot to investigate the effect of chronic bhang uptake on male reproductive physiology in mice
|Banerjee A,Singh A,Srivastava P,Turner H,Krishna A||Birth defects research. Part B, Developmental and reproductive toxicology (92:195)||2011|
Genetic and pharmacologic inactivation of cannabinoid CB1 receptor inhibits angiogenesis.
PA1-743 was used in western blot to investigate the importance of CB1 receptor in angiogenesis
|Pisanti S,Picardi P,Prota L,Proto MC,Laezza C,McGuire PG,Morbidelli L,Gazzerro P,Ziche M,Das A,Bifulco M||Blood (117:5541)||2011|
Levels of endocannabinoids and palmitoylethanolamide and their pharmacological manipulation in chronic granulomatous inflammation in rats.
PA1-743 was used in western blot to investigate the influence of endocannabinoids and palmitoylethanolamide on chronic granulomatous inflammation
|De Filippis D,D'Amico A,Cipriano M,Petrosino S,Orlando P,Di Marzo V,Iuvone T||Pharmacological research (61:321)||2010|
Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
PA1-743 was used in western blot to investigate the effect of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis
|Esposito G,Iuvone T,Savani C,Scuderi C,De Filippis D,Papa M,Di Marzo V,Steardo L||The Journal of pharmacology and experimental therapeutics (322:1144)||2007|