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|Tested species reactivity||Amphibian, Chicken, Fish, Human, Mouse, Primate, Rat|
|Published species reactivity||Rabbit, Ferret, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A fusion protein containing the first 77 amino acid residues from rat CB1.|
|Storage buffer||PBS with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-745 detects cannabinoid receptor 1 (CB1) from human, rat, monkey, mouse, chicken, salamander and goldfish tissues. This antibody detects a few non-specific bands in NIH-3T3 and HT-29 cell lysates.
PA1-745 has been successfully used in Western blot, FACS, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects a ~60 kDa protein representing CB1 and less intense bands of ~23 kDa, ~72 kDa, and ~180 kDa from rat brain homogenate. Immunocytochemical staining of CB1 in living or fixed cultured hippocampal neurons with PA1-745 results in intense, punctate staining along processes, with little staining over cell bodies.
PA1-745 antigen is a fusion protein containing the first 77 amino acid residues of the rat CB1 receptor.
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels.
CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells.
PA1-745 was used in flow cytometry and western blot to investigate the effects of proinflammatory cytokines on cannabinoid receptor expression and function
|Jean-Gilles L,Braitch M,Latif ML,Aram J,Fahey AJ,Edwards LJ,Robins RA,Tanasescu R,Tighe PJ,Gran B,Showe LC,Alexander SP,Chapman V,Kendall DA,Constantinescu CS||Acta physiologica (Oxford, England) (214:63)||2015|
Unbalance of CB1 receptors expressed in GABAergic and glutamatergic neurons in a transgenic mouse model of Huntington's disease.
PA1-745 was used in western blot to study the expression of CB1 receptors in GABAergic and glutamatergic neurons in a Huntington disease model
|Chiodi V,Uchigashima M,Beggiato S,Ferrante A,Armida M,Martire A,Potenza RL,Ferraro L,Tanganelli S,Watanabe M,Domenici MR,Popoli P||Neurobiology of disease (45:983)||2012|
Adenosine A2A receptors enable the synaptic effects of cannabinoid CB1 receptors in the rodent striatum.
PA1-745 was used in western blot to study the role of adenosine A2A receptors in the synaptic effects of cannabinoid CB1 receptors in the rodent striatum
|Tebano MT,Martire A,Chiodi V,Pepponi R,Ferrante A,Domenici MR,Frank C,Chen JF,Ledent C,Popoli P||Journal of neurochemistry (110:1921)||2009|
Effect of cannabidiol on sepsis-induced motility disturbances in mice: involvement of CB receptors and fatty acid amide hydrolase.
PA1-745 was used in western blot to evaluate cannabidiol as a potential therapeutic for sepsis-induced ileus
|de Filippis D,Iuvone T,d'amico A,Esposito G,Steardo L,Herman AG,Pelckmans PA,de Winter BY,de Man JG||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (20:919)||2008|
FGF-2 promotes neurogenesis and neuroprotection and prolongs survival in a transgenic mouse model of Huntington's disease.
PA1-745 was used in western blot to evaluate the neuroprotective and neuroproliferative effects of FGF-2 in a transgenic mouse model of Huntingdon disease
|Jin K,LaFevre-Bernt M,Sun Y,Chen S,Gafni J,Crippen D,Logvinova A,Ross CA,Greenberg DA,Ellerby LM||Proceedings of the National Academy of Sciences of the United States of America (102:18189)||2005|
Overexpression of cannabinoid CB2 receptor in the brain induces hyperglycaemia and a lean phenotype in adult mice.
PA1-745 was used in immunohistochemistry to study the effects of CB2 receptor overexpression in murine brain on body mass and glucose metabolism
|Romero-Zerbo SY,Garcia-Gutierrez MS,Suárez J,Rivera P,Ruz-Maldonado I,Vida M,Rodriguez de Fonseca F,Manzanares J,Bermúdez-Silva FJ||Journal of neuroendocrinology (24:1106)||2012|
CB1 and CB2 cannabinoid receptor expression during development and in epileptogenic developmental pathologies.
PA1-745 was used in immunohistochemistry to study the role of the CB1 and CB2 cannabinoid receptors in epileptogenic developmental pathologies
|Zurolo E,Iyer AM,Spliet WG,Van Rijen PC,Troost D,Gorter JA,Aronica E||Neuroscience (170:28)||2010|
Cannabinoid-1 (CB1) receptors regulate colonic propulsion by acting at motor neurons within the ascending motor pathways in mouse colon.
PA1-745 was used in immunohistochemistry to investigate the mechanism for the colonic propulsion mediated by cannabinoid-1 receptors
|Sibaev A,Yüce B,Kemmer M,Van Nassauw L,Broedl U,Allescher HD,Göke B,Timmermans JP,Storr M||American journal of physiology. Gastrointestinal and liver physiology (296:G119)||2009|
Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain.
PA1-745 was used in immunohistochemistry to characterize sn1-DAG lipases.
|Bisogno T,Howell F,Williams G,Minassi A,Cascio MG,Ligresti A,Matias I,Schiano-Moriello A,Paul P,Williams EJ,Gangadharan U,Hobbs C,Di Marzo V,Doherty P||The Journal of cell biology (163:463)||2003|
Delta9-tetrahydrocannabinol selectively acts on CB1 receptors in specific regions of dorsal vagal complex to inhibit emesis in ferrets.
PA1-745 was used in immunohistochemistry to investigate the antiemetic effect of THC in the ferret
|Van Sickle MD,Oland LD,Mackie K,Davison JS,Sharkey KA||American journal of physiology. Gastrointestinal and liver physiology (285:G566)||2003|
Effects of palmitoylation of Cys(415) in helix 8 of the CB(1) cannabinoid receptor on membrane localization and signalling.
PA1-745 was used in immunocytochemistry to investigate the role of CB(1) receptor palmitoylation in membrane localization and signalling
|Oddi S,Dainese E,Sandiford S,Fezza F,Lanuti M,Chiurchiù V,Totaro A,Catanzaro G,Barcaroli D,De Laurenzi V,Centonze D,Mukhopadhyay S,Selent J,Howlett AC,Maccarrone M||British journal of pharmacology (165:2635)||2012|
Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors.
PA1-745 was used in flow cytometry to investigate the mechanism of human T cell regulation by anandamide
|Cencioni MT,Chiurchiù V,Catanzaro G,Borsellino G,Bernardi G,Battistini L,Maccarrone M||PloS one (5:null)||2010|
brain-type cannabinoid receptor; cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB-R; CB1; central cannabinoid receptor; Cnr1; striatal cannabinoid receptor type 1 protein
CANN6; CB-R; CB1; CB1A; CB1K5; CB1R; CNR; CNR1; Skr6; SKR6R