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Western blot analysis of Cannabinoid Receptor II was performed by loading 25 ug of PC-3 (lane 1) and Jurkat (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Cannabinoid Receptor II polyclonal antibody (Product # PA1-744) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~40kDa.
|Tested species reactivity||Human|
|Published species reactivity||Primate, Hamster, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A fusion protein containing the first 33 amino acid residues from human CB2.|
|Storage buffer||PBS with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:100|
|Western Blot (WB)||1:250-1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-744 detects cannabinoid receptor 2 (CB2) from human tissues.
PA1-744 has been successfully used in Western blot, FACS, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa band from human spinal cord homogenate, and a ~40 kda band from RBL2H3 cells, representing the different glycosylated forms of CB2.
PA1-744 antigen is a fusion protein containing the first 33 amino acid residues from human CB2.
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels.
CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells.
PA1-744 was used in flow cytometry and western blot to investigate the effects of proinflammatory cytokines on cannabinoid receptor expression and function
|Jean-Gilles L,Braitch M,Latif ML,Aram J,Fahey AJ,Edwards LJ,Robins RA,Tanasescu R,Tighe PJ,Gran B,Showe LC,Alexander SP,Chapman V,Kendall DA,Constantinescu CS||Acta physiologica (Oxford, England) (214:63)||2015|
Characterization of peripheral human cannabinoid receptor (hCB2) expression and pharmacology using a novel radioligand, [35S]Sch225336.
PA1-744 was used in western blot to show that PA1-744 can not immunoreact with hCB2 successfully
|Gonsiorek W,Hesk D,Chen SC,Kinsley D,Fine JS,Jackson JV,Bober LA,Deno G,Bian H,Fossetta J,Lunn CA,Kozlowski JA,Lavey B,Piwinski J,Narula SK,Lundell DJ,Hipkin RW||The Journal of biological chemistry (281:28143)||2006|
Cannabinoid receptor CB2 is expressed on vascular cells, but not astroglial cells in the post-mortem human Huntington's disease brain.
PA1-744 was used in immunocytochemistry and immunohistochemistry to study the expressino of cannabinoid receptor CB2 on vascular and astroglial cells in the post-mortem Huntington disease brain
|Dowie MJ,Grimsey NL,Hoffman T,Faull RL,Glass M||Journal of chemical neuroanatomy (59-60:62)||2014|
Cannabinoids and bone: endocannabinoids modulate human osteoclast function in vitro.
PA1-744 was used in immunocytochemistry to investigate the effect of the endocannabinoids on human osteoclast function in vitro
|Whyte LS,Ford L,Ridge SA,Cameron GA,Rogers MJ,Ross RA||British journal of pharmacology (165:2584)||2012|
The peripheral cannabinoid receptor Cb2, frequently expressed on AML blasts, either induces a neutrophilic differentiation block or confers abnormal migration properties in a ligand-dependent manner.
PA1-744 was used in immunocytochemistry to study the mechanism of Cb2-mediated differentiation block.
|Alberich Jordà M,Rayman N,Tas M,Verbakel SE,Battista N,van Lom K,Löwenberg B,Maccarrone M,Delwel R||Blood (104:526)||2004|
The expression of the peripheral cannabinoid receptor CB2 has no effect on clinical outcome in diffuse large B-cell lymphomas.
PA1-744 was used in immunohistochemistry to study the influence of cannabinoid receptor CB2 expression on clinical outcomes of lymphomas
|Rayman N,Lam KH,van der Holt B,Koss C,van Leeuwen J,Budel LM,Mulder AH,Sonneveld P,Delwel R||European journal of haematology (86:466)||2011|
A glial endogenous cannabinoid system is upregulated in the brains of macaques with simian immunodeficiency virus-induced encephalitis.
PA1-744 was used in immunohistochemistry to investigate the role of cannabinoid receptors during the development of human immunodeficiency virus-induced encephalitis.
|Benito C,Kim WK,Kim WK,Chavarría I,Hillard CJ,Mackie K,Tolón RM,Williams K,Williams K,Romero J||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:2530)||2005|
Distinct expression profiles of the peripheral cannabinoid receptor in lymphoid tissues depending on receptor activation status.
PA1-744 was used in immunohistochemistry to study the role of CB2 in the migration and activation of B cells.
|Rayman N,Lam KH,Laman JD,Simons PJ,Löwenberg B,Sonneveld P,Delwel R||Journal of immunology (Baltimore, Md. : 1950) (172:2111)||2004|
Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.
PA1-744 was used in immunohistochemistry to study the expression of cannabinoid CB2 receptors and fatty acid amide hydrolase in neuritic plaque-associated glia in Alzheimer disease brains.
|Benito C,Núñez E,Tolón RM,Carrier EJ,Rábano A,Hillard CJ,Romero J||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:11136)||2003|
cannabinoid receptor 2 (macrophage); CB2; Cnr2; testis-dominant CNR2 isoform CB2
CB-2; CB2; CB2A; CB2B; CNR2; CX5