Invitrogen

Carbonic Anhydrase IV Polyclonal Antibody

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Carbonic Anhydrase IV Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47312) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells.

Carbonic Anhydrase IV Antibody in Western Blot (WB) — Western blot analysis from lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47312) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.

Carbonic Anhydrase IV Antibody in Immunohistochemistry (IHC) — Figure 4 Altered EC gene expression in the ECs of Dll4 +/lacZ mice. ( A ) Whole-lung RNA homogenates obtained from Dll4 +/+ and Dll4 +/lacZ at P4 and P28 were used to quantify Kdr, Jag1, Nr2f2, Ephb4, Vegfa, Car4, Esm1, Apln, and Aplnr with qRT-PCR. n = 5 mice per group; * P < 0.05, ** P < 0.01, *** P < 0.001. ( B ) Mouse lung ECs (PECAM pulldown) obtained from Dll4 +/+ and Dll4 +/lacZ at P6 and P14 were used to quantify Dll4, Nrp1, Jag1, Car4, Apln, Esm1, Efnb2, and Aplnr . n = 5 mice per group; * P < 0.01. ( C ) Mouse lung ECs were harvested from 14-day-old Dll4 +/+ and Dll4 +/lacZ mice. Lung homogenates were used to quantify DLL4, NICD, HES1, JAG1, VEGFA, and PECAM by immunoblotting, with densitometry shown graphically ( D ). n = 5 mice per group; * P < 0.01. ( E ) PECAM (green), CAR4 (red), and DAPI staining on P14 Dll4 +/+ and Dll4 +/lacZ lung sections ( n = 4). ( F ) PECAM (green), APLNR (red) and DAPI (blue) staining on Dll4 +/+ and Dll4 +/lacZ mouse lung slides ( n = 4). Scale bars: 10 mum. Data are shown as mean +- SD. ( A , B , and D , Gaussian distribution used 1-way ANOVA with Tukey''s test) ( A and B ) Non-Gaussian distribution used 2-tailed Mann-Whitney U test.

Product Details

PA5-47312

Applications	Tested Dilution	Publications
Western Blot (WB)	0.25-12.5 µg/mL	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	5-15 µg/mL	-
Immunoprecipitation (IP)	25 µg/mL	-

Product Specifications
Species Reactivity	Mouse
Published species	Not Applicable
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4 Glu18-Ser277 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2607329

Product Specific Information

In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) CA4 is observed and less than 2% cross-reactivity with rhCA3, rhCA10, rhCA13, recombinant mouse (rm) CA9, and rmCA12 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. Its exact function is not known, however, it may have a role in inherited renal abnormalities of bicarbonate transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.