Invitrogen

Carbonic Anhydrase IV Polyclonal Antibody

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Carbonic Anhydrase IV Antibody in Western Blot (WB) — Western blot analysis from lysates of mouse lung tissue and mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 51-52 kDa (as indicated) using 12.5 µg/mL of Goat Anti-mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47312)

Carbonic Anhydrase IV Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47312) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-12.5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

Immunoprecipitation (IP)

25 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4 Glu18-Ser277

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2607329

Product Specific Information

In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) CA4 is observed and less than 2% cross-reactivity with rhCA3, rhCA10, rhCA13, recombinant mouse (rm) CA9, and rmCA12 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. Its exact function is not known, however, it may have a role in inherited renal abnormalities of bicarbonate transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.