Immunofluorescent analysis of Carboxylesterase 2 showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in ice-cold MeOH for 5 min and stained using a Carboxylesterase 2 polyclonal antibody (Product # PA5-28027) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 51 and 469 of Human Carboxylesterase 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28027 targets Carboxylesterase 2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28027 immunogen is recombinant fragment corresponding to a region within amino acids 51 and 469 of Human Carboxylesterase 2.
Carboxylesterase 2 is a member of a large multigene family. The enzymes encoded by these genes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. The specific function of this enzyme has not yet been determined; however, it is speculated that carboxylesterases may play a role in lipid metabolism and/or the blood-brain barrier system. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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