Western blot detection of Carboxypeptidase Y in 100ng per lane of (lane 1) Carboxypeptidase Y and (lane 2) control with no antibody using a Carboxypeptidase Y polyclonal antibody (Product # PA1-27244) at a dilution of 1:1000 for 60 min at room temperature, block for 30 min at room temperature.
|Tested species reactivity||Yeast|
|Published species reactivity||Fungi|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Carboxypeptidase Y from S. cerevisiae.|
|Storage buffer||PBS, pH 7.2, with 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||Assay Dependent|
|ELISA (ELISA)||1:20,000 - 100,000|
|Immunomicroscopy (IM)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27244 detects Carboxypeptidase Y from S. cerevisiae samples.
PA1-27244 has been successfully used in Western blot, ELISA, Immunomicroscopy, and Dot blot procedures.
The PA1-27244 immunogen is Carboxypeptidase Y from S. cerevisiae.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An essential role for phosphatidylinositol 3-kinase in the inhibition of phagosomal maturation, intracellular survival and virulence in Candida glabrata.
PA1-27244 was used in western blot to investigate the contribution of the Candida glabrata CgVPS15 gene to phagocytosis
|Rai MN,Sharma V,Balusu S,Kaur R||Cellular microbiology (17:269)||2015|
GPI (glycosylphosphatidylinositol)-linked aspartyl proteases regulate vacuole homoeostasis in Candida glabrata.
PA1-27244 was used in western blot to study the role of Candida glabrata GPI-anchored yapsin aspartyl proteinases in the modulation of vacuolar homeostasis
|Bairwa G,Rasheed M,Taigwal R,Sahoo R,Kaur R||The Biochemical journal (458:323)||2014|