Western blot analysis of Cardiac Troponin I in 3 ng (Lane 1) 10 ng (Lane 2) 30 ng (Lane 3) and 100 ng (Lane 4) cTNI using a Cardiac Troponin I monoclonal antibody (Product # MA1-20112). Detection was performed using an HRP-conjugated goat anti-mouse IgG antibody and diaminobenzidine substrate.
|Tested species reactivity||Bovine, Dog, Cat, Goat, Human, Mouse, Pig, Rabbit, Rat|
|Published species reactivity||Zebrafish|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length, human cardiac troponin 1 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
MA1-20112 detects cardiac troponin 1 in human, mouse, rat, bovine, feline, canine, goat, porcine and rabbit samples.
MA1-20112 has been successfully used in ELISA and Western blot procedures.
The MA1-20112 immunogen is full length, human cardiac troponin 1 protein.
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. It also binds to actin and tropomyosin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased radial glia quiescence, decreased reactivation upon injury and unaltered neuroblast behavior underlie decreased neurogenesis in the aging zebrafish telencephalon.
MA1-20112 was used in immunohistochemistry to investigate the effect of aging on neurogenesis and its mechanism in zebrafish telencephalon
|Edelmann K,Glashauser L,Sprungala S,Hesl B,Fritschle M,Ninkovic J,Godinho L,Chapouton P||The Journal of comparative neurology (521:3099)||2013|
The cellular composition of neurogenic periventricular zones in the adult zebrafish forebrain.
MA1-20112 was used in immunohistochemistry to investigate neurogenic periventricular zones in the adult zebrafish forebrain
|Lindsey BW,Darabie A,Tropepe V||The Journal of comparative neurology (520:2275)||2012|
Neuronal and glial localization of alpha(2A)-adrenoceptors in the adult zebrafish (Danio rerio) brain.
MA1-20112 was used in immunohistochemistry to localize alpha(2A)-adrenoceptors in the adult zebrafish brain
|Ampatzis K,Kentouri M,Dermon CR||The Journal of comparative neurology (508:72)||2008|