Western blot of Cardiolipin Synthase 1 in HEK293 lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a Cardiolipin Synthase 1 polyclonal antibody (Product# PA5-37950) at a dilution of 0.2ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-RSALGSALDPLADK, from the internal region of CRLS1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.2-0.6 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, dog, pig and cow based on sequence homology.
The function of this protein is still unknown. The gene of human hypothetical protein (LOC54675) is up-regulated in colon cancer metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.