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|Tested species reactivity||Dog, Cat, Goat, Hamster, Human, Mouse, Primate, Rabbit, Rat, Clawed frog|
|Host / Isotype||Mouse / IgM|
|Immunogen||Total protein extract of chicken gizzard.|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:100|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:25 - 100|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OMA1-06030 detects cardiotin from mouse, rat, hamster, canine, monkey, pig, cat, rabbit, frog, goat, xenopus,feline and human cardiomyocytes.
OMA1-06030 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~300 kDa protein representing the cardiotin protein complex, as well as ~100 kDa and ~60 kDa bands representing the cardiotin subunits from rat heart extract.
The OMA1-06030 immunogen is total protein extract from chicken gizzard.
Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.