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|Tested species reactivity||Human, Pig|
|Host / Isotype||Mouse / IgM|
|Immunogen||100 kDa cardiotin subunit.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:100|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06308 detects cardiotin in human and porcine samples.
MA1-06308 has sucessfully been used in Western blotting and immunohistochemistry. By Western blotting, it detects a ~300 kDa protein representing cardiotin.
The MA1-06308 immunogen is the ~100 kDa cardiotin subunit.
Cardiotin is a high molecular weight protein complex (~300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of ~60 kDa and ~100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.