Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
Cardiotrophin 1 Antibody (500-P101-100UG) in WB
Western Blot: To detect Human Cardiotrophin-1 by Western Blot analysis Cardiotrophin 1 Polyclonal Antibody (Product # 500-P101-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Cardiotrophin-1 is 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Product Details
500-P101-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Cardiotrophin-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929184
Product Specific Information
AA Sequence of recombinant protein: SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Cardiotrophin-1. Anti-Human Cardiotrophin-1-specific antibody was purified by affinity chromatography employing an immobilized Human Cardiotrophin-1 matrix.
Sandwich ELISA: To detect Human Cardiotrophin-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Cardiotrophin-1 (500-P101Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Cardiotrophin-1.
Western Blot: To detect Human Cardiotrophin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Cardiotrophin-1 is 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Target Information
Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and has been shown to induce hypertrophy in cardiac myocytes in vitro. Cardiotrophin-1, a secreted protein expressed at high levels in myocardium during cardiogenesis, has been shown to promote proliferation and survival of embryonic cardiomyocytes, suggesting a role for Cardiotrophin-1 in the activation of gp130 during cardiac development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cardiophin 1; Cardiotrophin; Cardiotrophin-1; Cardiotrophin1; CT 1; CT-1; CT-1CT1; CTF 1
Gene Aliases: CT-1; CT1; CTF1
UniProt ID: (Human) Q16619
Entrez Gene ID: (Human) 1489
cell surface receptor signaling pathway
cell-cell signaling
nervous system development
muscle organ development
cell proliferation
positive regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat3 protein
neuron development
leukemia inhibitory factor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support