Immunofluorescent analysis of Casein Kinase 1 alpha 1L showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a Casein Kinase 1 alpha 1L polyclonal antibody (Product # PA5-28272) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 10 and 228 of Human Casein Kinase 1 alpha 1L|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28272 targets Casein Kinase 1 alpha 1L in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28272 immunogen is recombinant fragment corresponding to a region within amino acids 10 and 228 of Human Casein Kinase 1 alpha 1L.
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling.
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