Immunofluorescent analysis of Casein Kinase 1 delta in paraformaldehyde-fixed HeLa cells using a Casein Kinase 1 delta polyclonal antibody (Product # PA5-32129) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 144 and 361 of Human Casein Kinase 1 delta|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32129 targets Casein Kinase 1 delta in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. Predicted to react with Rat and Bovine based on 100% sequence homology.
The PA5-32129 immunogen is recombinant protein fragment corresponding to a region within amino acids 144 and 361 of Human Casein Kinase 1 delta.
This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.