Immunofluorescent analysis of Casein Kinase 2 alpha 1 showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Casein Kinase 2 alpha 1 polyclonal antibody (Product # PA5-28686) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 166 and 360 of Human Casein Kinase 2 alpha 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-28686 targets Casein Kinase 2 alpha 1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28686 immunogen is recombinant protein fragment corresponding to a region within amino acids 166 and 360 of Human Casein Kinase 2 alpha 1.
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice.
PA5-28686 was used in immunocytochemistry to study Brd4 function in the brain
|Korb E,Herre M,Zucker-Scharff I,Darnell RB,Allis CD||Nature neuroscience (18:1464)||2015|