Immunohistochemical analysis of Caspase-1 using a Caspase-1 monoclonal antibody (Product # MA5-16215). Formalin-fixed, paraffin-embedded sections of human colon cancer stained for Caspase-1 expression using a DAB chromogen and Hematoxylin counterstain. Nine sections, from different human patients were stained. Differential staining was observed (top right panel). Two of these sections are shown at higher magnifications (arrows and A, B). Only rare staining in A is observed. In contrast, abundant staining is observed in B.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide (aa 371-390) of Caspase-1 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: 3T3 whole cell lysate, antigen standard for CASP1 (transient overexpression lysate), HeLa or NIH-3T3, Hela whole cell lysate.
Caspase proteins are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspase proteins is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1 (ICE, IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.