|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (PEHKTSDSTFLVFMSHGIREG) corresponding to amino acids 129-149 of human Caspase-1, coupled to KLH.|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10 µg/mL|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||0.5-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Hypoxia primes human normal prostate epithelial cells and cancer cell lines for the NLRP3 and AIM2 inflammasome activation.
AHZ0082 was used in western blot to assess the priming of human normal prostate epithelial cells and cancer cell lines for the NLRP3 and AIM2 inflammasome activation due to hypoxia
|Panchanathan R,Liu H,Choubey D||Oncotarget (7:28183)||2016|
Chemotherapy-triggered cathepsin B release in myeloid-derived suppressor cells activates the Nlrp3 inflammasome and promotes tumor growth.
AHZ0082 was used in western blot to study the effect of gemcitabine and 5-fluorouracil treatment on myeloid-derived suppressor cells.
|Bruchard M,Mignot G,Derangère V,Chalmin F,Chevriaux A,Végran F,Boireau W,Simon B,Ryffel B,Connat JL,Kanellopoulos J,Martin F,Rébé C,Apetoh L,Ghiringhelli F||Nature medicine (19:57)||2013|
|Mouse||Not Cited||Aim2 deficiency stimulates the expression of IFN-inducible Ifi202, a lupus susceptibility murine gene within the Nba2 autoimmune susceptibility locus.||Panchanathan R,Duan X,Shen H,Rathinam VA,Erickson LD,Fitzgerald KA,Choubey D||Journal of immunology (Baltimore, Md. : 1950) (185:7385)||2010|
|Mouse||Not Cited||Caspase-1 and -3 are sequentially activated in motor neuron death in Cu,Zn superoxide dismutase-mediated familial amyotrophic lateral sclerosis.||Pasinelli P,Houseweart MK,Brown RH,Cleveland DW||Proceedings of the National Academy of Sciences of the United States of America (97:13901)||2000|
Suppression in PHLPP2 induction by morin promotes Nrf2-regulated cellular defenses against oxidative injury to primary rat hepatocytes.
AHZ0082 was used in western blot to examine the Nrf2-potentiating mechanism of morin and its possible role in intervening PHLPP2-regulated Akt/GSK3β/Fyn kinase axis.
|Rizvi F,Mathur A,Krishna S,Siddiqi MI,Kakkar P||Redox biology (6:587)||2015|
||Aim2 deficiency stimulates the expression of IFN-inducible Ifi202, a lupus susceptibility murine gene within the Nba2 autoimmune susceptibility locus.||Panchanathan R,Duan X,Shen H,Rathinam VA,Erickson LD,Fitzgerald KA,Choubey D||Journal of immunology (Baltimore, Md. : 1950) (185:7385)||2010|