Invitrogen

Caspase 1 Monoclonal Antibody (5B10), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

18 References

View all (24) Caspase 1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Caspase 1 Antibody in Western Blot (WB) — Lysates prepared from the Jurkat (lane 1) and J774 (lane 2) cell lines under reducing conditions with DTT were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Mouse Caspase 1 Purified. Bands were visualized using Anti-Rat IgG HRP.

Caspase 1 Antibody in Flow Cytometry (Flow) — Figure 7. Mitochondrial Stress Triggers Caspase 1 Activation in Aged HSCs (A and B) Aged HSCs were transduced with indicated lentivirus: SIRT3, SIRT7, SOD2KR (A) and NRF1 shRNA(B). At 2 days post-transduction, cells were stained for activated caspase 1 and analyzed via flow cytometry. Data shown are activated caspase 1 + cells in the gated HSC population. n = 3. (C and D) Bone marrow cells of SIRT7 KO mice and their WT controls were stained for activated caspases. Data shown are the percentage of caspase + cells in HSCs. n = 5 and 6 (C). n = 5 (D). (E) A proposed model. SIRT2 represses NLRP3 inflammasome activation in HSCs. In aged HSCs, reduced SIRT2 expression and increased mitochondrial stresses lead to activation of the NLRP3 inflammasome, reduced HSC survival, and reduced functionality. Error bars represent SE. *p < 0.05. **p < 0.01. ns, p > 0.05. Student's t test.

Caspase 1 Antibody — Figure 6 F991 inhibited inflammasome activation and inflammatory cell infiltration in the AOM/DSS-induced CAC model. ( A ) Levels of active IL-1beta, IL-18, pro-caspase-1, cleaved caspase-1 (p20) and ASC in the colonic tissues were determined by western blot. Data were from 4 mice were shown. ( B ) CD45 + cell, ( C ) mast cells, macrophages, neutrophils and ( D ) subpopulations of DCs were detected using flow cytometry. The results ( B - D ) are shown as the mean +- SD of 10 mice per group. ns: no statistical significance , * P < 0.05 and ** P < 0.01 , compared with the group AOM + DSS + PBS. AOM + DSS + PBS : AOM/DSS-induced CAC mice and injected with PBS, AOM + DSS + F991 : AOM/DSS-induced CAC mice and injected with F991. One representative experiment of two is displayed. {TM}

Product Details

14-9832-82

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	View 14 publications 14 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	5B10
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_2016691

Product Specific Information

Description: The monoclonal antibody 5B10 recognizes mouse Caspase-1, a member of the cysteine-requiring aspartate protease family, which plays a critical role in cell suicide known as apoptosis. In particular, it converts pro-IL-1beta to mature IL-1beta; hence it is also called ICE (interleukin-1beta converting enzyme). Upon activation Caspase-1 exists as a tetramer of two p20 and two p10 subunits. There are several isoforms of Caspase-1.

The 5B10 antibody recognizes both the pro-form of about 42kDa and the active p20 form.

Applications Reported: This 5B10 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 5B10 antibody has been tested by western blot analysis of lysates prepared from the J774 cell line. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glycosylation-Dependent Induction of Programmed Cell Death in Murine Adenocarcinoma Cells.

Frontiers in immunology

Parshenkov A,Hennet T

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Western Blot

Fri Apr 08 00:00:00 EDT 2022

Modulation of the NLRP3 inflammasome by Sars-CoV-2 Envelope protein.

Scientific reports

Yalcinkaya M,Liu W,Islam MN,Kotini AG,Gusarova GA,Fidler TP,Papapetrou EP,Bhattacharya J,Wang N,Tall AR

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Western Blot

Fri Dec 24 00:00:00 EST 2021

NLPR3 inflammasome inhibition alleviates hypoxic endothelial cell death in vitro and protects blood-brain barrier integrity in murine stroke.

Cell death & disease

Bellut M,Papp L,Bieber M,Kraft P,Stoll G,Schuhmann MK

14-9832-82 was used in Immunohistochemistry (Frozen) to study the survival of brain endothelial cells exposed to oxygen and glucose deprivation in vitro in the context of inflammasome inhibition with the NLR-family pyrin domain-containing protein 3 (NLRP3)-specific drug MCC950.

Mon Dec 20 00:00:00 EST 2021

Integrative metabolomics and transcriptomics identifies itaconate as an adjunct therapy to treat ocular bacterial infection.

Cell reports. Medicine

Singh S,Singh PK,Jha A,Naik P,Joseph J,Giri S,Kumar A

14-9832-82 was used in Western Blot to study the anti-inflammatory effects of itaconate using integrated metabolomics and transcriptomics.

Tue May 18 00:00:00 EDT 2021

Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting.

Nature communications

Brocker CN,Kim D,Melia T,Karri K,Velenosi TJ,Takahashi S,Aibara D,Bonzo JA,Levi M,Waxman DJ,Gonzalez FJ

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Western Blot

Tue Nov 17 00:00:00 EST 2020

Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting.

Nature communications

Brocker CN,Kim D,Melia T,Karri K,Velenosi TJ,Takahashi S,Aibara D,Bonzo JA,Levi M,Waxman DJ,Gonzalez FJ

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Western Blot

Tue Nov 17 00:00:00 EST 2020

Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis.

Immunity

Tummers B,Mari L,Guy CS,Heckmann BL,Rodriguez DA,Rühl S,Moretti J,Crawford JC,Fitzgerald P,Kanneganti TD,Janke LJ,Pelletier S,Blander JM,Green DR

14-9832-82 was used in Western Blot to suggest an in vivo role of the inflammatory RIPK1-caspase-8-FADD (FADDosome) complex and reveal a FADD-independent inflammatory role of caspase-8 that involves activation of an inflammasome.

Tue Jun 16 00:00:00 EDT 2020

An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance.

Cell metabolism

He M,Chiang HH,Luo H,Zheng Z,Qiao Q,Wang L,Tan M,Ohkubo R,Mu WC,Zhao S,Wu H,Chen D

14-9832 was used in Western Blotting to establish the dysregulation of the acetylation switch of the NLRP3 inflammasome as an origin of aging-associated chronic inflammation and highlight the reversibility of aging-associated chronic inflammation and insulin resistance.

Tue Mar 03 00:00:00 EST 2020

Cutting Edge: TAK1 Safeguards Macrophages against Proinflammatory Cell Death.

Journal of immunology (Baltimore, Md. : 1950)

Sanjo H,Nakayama J,Yoshizawa T,Fehling HJ,Akira S,Taki S

14-9832-82 was used in Cell Culture to reveal that TAK1 acts as a signaling checkpoint to protect macrophages from unique proinflammatory cell death, ensuring the maintenance of innate immune homeostasis.

Thu Aug 15 00:00:00 EDT 2019

Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging.

Cell reports

Luo H,Mu WC,Karki R,Chiang HH,Mohrin M,Shin JJ,Ohkubo R,Ito K,Kanneganti TD,Chen D

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Flow Cytometry

Tue Jan 22 00:00:00 EST 2019

Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging.

Cell reports

Luo H,Mu WC,Karki R,Chiang HH,Mohrin M,Shin JJ,Ohkubo R,Ito K,Kanneganti TD,Chen D

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Flow Cytometry

Tue Jan 22 00:00:00 EST 2019

Free immunoglobulin light chain (FLC) promotes murine colitis and colitis-associated colon carcinogenesis by activating the inflammasome.

Scientific reports

Ma J,Jiang D,Gong X,Shao W,Zhu Z,Xu W,Qiu X

14-9832 was used in Western Blotting to investigate whether free Ig light chain is involved in colitis and colitis-associated colon carcinogenesis.

Wed Jul 12 00:00:00 EDT 2017

Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.

Nature medicine

Lood C,Blanco LP,Purmalek MM,Carmona-Rivera C,De Ravin SS,Smith CK,Malech HL,Ledbetter JA,Elkon KB,Kaplan MJ

14-9832 was used in Western Blotting to investigate how neutrophil extracellular traps are generated and their roles in sterile inflammation.

Mon Feb 01 00:00:00 EST 2016

Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants.

The Journal of biological chemistry

Marty-Roix R,Vladimer GI,Pouliot K,Weng D,Buglione-Corbett R,West K,MacMicking JD,Chee JD,Wang S,Lu S,Lien E

14-9832 was used in Western Blotting to determine the mechanisms of cellular action induced by QS-21.

Fri Jan 15 00:00:00 EST 2016

Bruton's tyrosine kinase is essential for NLRP3 inflammasome activation and contributes to ischaemic brain injury.

Nature communications

Ito M,Shichita T,Okada M,Komine R,Noguchi Y,Yoshimura A,Morita R

14-9832 was used in Immunoprecipitation to report that Bruton's tyrosine kinase (BTK) is an essential component of the NLRP3 inflammasome, in which BTK physically interacts with ASC and NLRP3.

Wed Jun 10 00:00:00 EDT 2015

Synthetic oligodeoxynucleotides containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation.

Journal of immunology (Baltimore, Md. : 1950)

Kaminski JJ,Schattgen SA,Tzeng TC,Bode C,Klinman DM,Fitzgerald KA

14-9832 was used in Western Blotting to investigate the effect of ODN-A151 in dendritic cells and macrophages.

Tue Oct 01 00:00:00 EDT 2013

Nitric oxide controls the immunopathology of tuberculosis by inhibiting NLRP3 inflammasome-dependent processing of IL-1β.

Nature immunology

Mishra BB,Rathinam VA,Martens GW,Martinot AJ,Kornfeld H,Fitzgerald KA,Sassetti CM

14-9832 was used in Western Blotting to investigate the mechanisms that controls IL-1 during tuberculosis.

Tue Jan 01 00:00:00 EST 2013

TRIF licenses caspase-11-dependent NLRP3 inflammasome activation by gram-negative bacteria.

Cell

Rathinam VA,Vanaja SK,Waggoner L,Sokolovska A,Becker C,Stuart LM,Leong JM,Fitzgerald KA

14-9832 was used in Western Blotting to investigate the molecular mechanisms underlying "sepsis syndrome" response during systemic Gram-negative bacteria infection.

Fri Aug 03 00:00:00 EDT 2012

Chitosan but not chitin activates the inflammasome by a mechanism dependent upon phagocytosis.

The Journal of biological chemistry

Bueter CL,Lee CK,Rathinam VAK,Healy GJ,Taron CH,Specht CA,Levitz SM

14-9832 was used in Western Blotting to investigate the capacity of chitin to stimulate murine macrophages to release the inflammasome-associated cytokine IL-1β.

Fri Oct 14 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CASP-1; Caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; Caspase1; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; interleukin 1 beta-converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

Gene Aliases: Casp1; ICE; Il1bc

UniProt ID: (Mouse) P29452

Entrez Gene ID: (Mouse) 12362

Molecular Function: protease