Invitrogen

Caspase 1 Monoclonal Antibody (5B10), eBioscience™

Product Image Gallery

Caspase 1 Antibody — Figure 3 F991 relieved inflammasome activation in the inflammatory tissue of DSS-induced colitis mice. ( A ) The level of IL-6, TNFalpha, IL-10 in the cultured supernatant of colonic tissue on the 2 nd , 4 th and 6 th day were examined by ELISA. The results are shown as the mean +- SD of 4 mice per group at each time point. ns: no statistical significance , * P < 0.05 , ** P < 0.01 , *** P < 0.001 compared with group DSS + PBS. ( B ) The level of active IL-1beta, IL-18, pro-caspase-1, cleaved caspase-1 (p20) and ASC in the colonic tissues on the 6 th day were determined by western blot. ( C ) The level of phosphorylated p65 (p-p65), and phosphorylated-STAT3 (p-STAT3) in the colonic tissues on the 6 th day were analyzed by western blot. DSS + PBS : mice challenged with DSS and injected with PBS, DSS + F991 : mice challenged with DSS and injected with F991. One representative experiment of three is displayed.

Caspase 1 Antibody in Western Blot (WB) — Lysates prepared from the Jurkat (lane 1) and J774 (lane 2) cell lines under reducing conditions with DTT were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Mouse Caspase 1 Purified. Bands were visualized using Anti-Rat IgG HRP.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1-5 µg/mL

Published Applications

Western Blot (WB)

See 6 publications below

Immunocytochemistry (ICC)

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host / Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5B10

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_2016691

Product Specific Information

Description: The monoclonal antibody 5B10 recognizes mouse Caspase-1, a member of the cysteine-requiring aspartate protease family, which plays a critical role in cell suicide known as apoptosis. In particular, it converts pro-IL-1beta to mature IL-1beta; hence it is also called ICE (interleukin-1beta converting enzyme). Upon activation Caspase-1 exists as a tetramer of two p20 and two p10 subunits. There are several isoforms of Caspase-1.

The 5B10 antibody recognizes both the pro-form of about 42kDa and the active p20 form.

Applications Reported: This 5B10 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 5B10 antibody has been tested by western blot analysis of lysates prepared from the J774 cell line. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CASP-1; Caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1BC; interleukin 1 beta-converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; P45

Gene Aliases: Casp1; ICE; Il1bc

UniProt ID: (Mouse) P29452

Entrez Gene ID: (Mouse) 12362

Molecular Function: cysteine protease enzyme modulator hydrolase protease protease inhibitor