Proteintech

Caspase 1/p20/p10 Polyclonal Antibody

33 References

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Protocols

Questions & Answers

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Caspase 1/p20/p10 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of C6 cells using 22915-1-AP (Caspase 1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Caspase 1/p20/p10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen tissue slide using 22915-1-AP ( Caspase 1 Antibody) at dilution of 1:200 (under 40x lens).

Caspase 1/p20/p10 Antibody in Western Blot (WB) — MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (CASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

22915-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 29 publications 29 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Bovine, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 1/p20/p10 Fusion Protein Ag11143 (1-311 aa encoded by BC062327) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.35 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

22915-1-AP can recognize all five isoforms of caspase 1. It also recognizes p45 procaspase and the mature fragments p10 and p20.

Immunogen sequence: MADKVLKEK RKLFIRSMGE APQAVQDNPA MPTSSGSEGN VKLCSLEEAQ RIWKQKSAEI YPIMDKSSRT RLALIICNEE FDSIPRRTGA EVDITGMTML LQNLGYSVDV KKNLTASDMT TELEAFAHRP EHKTSDSTFL VFMSHGIREG ICGKKHSEQV PDILQLNAIF NMLNTKNCPS LKDKPKVIII QACRGDSPGV VWFKDSVGVS GNLSLPTTEE FEDDAIKKAH IEKDFIAFCS STPDNVSWRH PTMGSVFIGR LIEHMQEYAC SCDVEEIFRK VRFSFEQPDG RAQMPTTERV TLTRCFYLFP GH (1-311 aa encoded by BC062327)

Target Information

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Du Y,Gu X,Meng H,Aa N,Liu S,Peng C,Ge Y,Yang Z

Wed Sep 30 00:00:00 EDT 2020

Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Activation of NLRP3 and NLRC4 Inflammasomes With Differential Requirement for ASC.

Frontiers in microbiology

Wu Q,Zhu YH,Xu J,Liu X,Duan C,Wang MJ,Wang JF

Wed Sep 30 00:00:00 EDT 2020

Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Activation of NLRP3 and NLRC4 Inflammasomes With Differential Requirement for ASC.

Frontiers in microbiology

Wu Q,Zhu YH,Xu J,Liu X,Duan C,Wang MJ,Wang JF

Wed Sep 30 00:00:00 EDT 2020

Methylene Blue Attenuates Diabetic Retinopathy by Inhibiting NLRP3 Inflammasome Activation in STZ-Induced Diabetic Rats.

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Hao J,Zhang H,Yu J,Chen X,Yang L

Tue Jan 07 00:00:00 EST 2020

Verapamil Ameliorates Hepatic Metaflammation by Inhibiting Thioredoxin-Interacting Protein/NLRP3 Pathways.

Frontiers in endocrinology

Zhou F,Zhang Y,Chen J,Hu Y,Xu Y

Wed Nov 20 00:00:00 EST 2019

OxLDL induces vascular endothelial cell pyroptosis through miR-125a-5p/TET2 pathway.

Journal of cellular physiology

Zhaolin Z,Jiaojiao C,Peng W,Yami L,Tingting Z,Jun T,Shiyuan W,Jinyan X,Dangheng W,Zhisheng J,Zuo W

Wed May 01 00:00:00 EDT 2019

OxLDL induces vascular endothelial cell pyroptosis through miR-125a-5p/TET2 pathway.

Journal of cellular physiology

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Role of Thymoquinone in Cardiac Damage Caused by Sepsis from BALB/c Mice.

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Liu H,Sun Y,Zhang Y,Yang G,Guo L,Zhao Y,Pei Z

Mon Apr 01 00:00:00 EDT 2019

Role of Thymoquinone in Cardiac Damage Caused by Sepsis from BALB/c Mice.

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Mon Apr 01 00:00:00 EDT 2019

Porous Se@SiO₂ nanospheres attenuate ischemia/reperfusion (I/R)-induced acute kidney injury (AKI) and inflammation by antioxidative stress.

International journal of nanomedicine

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Tue Feb 12 00:00:00 EST 2019

Involvement of NLRP3 inflammasome in methamphetamine-induced microglial activation through miR-143/PUMA axis.

Toxicology letters

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Fri Feb 01 00:00:00 EST 2019

Granisetron protects polymicrobial sepsis-induced acute lung injury in mice.

Biochemical and biophysical research communications

Wang J,Gong S,Wang F,Niu M,Wei G,He Z,Gu T,Jiang Y,Liu A,Chen P

Tue Jan 22 00:00:00 EST 2019

LncRNA XIST mediates bovine mammary epithelial cell inflammatory response via NF-κB/NLRP3 inflammasome pathway.

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Oncology reports

Zhang Q,Tao X,Xia S,Qu J,Song H,Liu J,Li H,Shang D

Tue Jan 01 00:00:00 EST 2019

Salidroside Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease via AMPK-Dependent TXNIP/NLRP3 Pathway.

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Genipin Reverses HFD-Induced Liver Damage and Inhibits UCP2-Mediated Pyroptosis in Mice.

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Song S,Qiu D,Luo F,Wei J,Wu M,Wu H,Du C,Du Y,Ren Y,Chen N,Duan H,Shi Y

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2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside attenuates methionine and choline-deficient diet-induced non-alcoholic fatty liver disease.

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Bioinformatics

Protein Aliases: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45

UniProt ID: (Human) Q6DMQ1, (Mouse) P29452

Entrez Gene ID: (Human) 834, (Mouse) 12362, (Rat) 25166

Molecular Function: protease