This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details
Description: The monoclonal antibody 17D9 recognizes mouse Caspase-11, a cysteine protease that plays a role in apoptosis and inflammation. Caspase-11 exists as a 43 kDa and 38 kDa pro-caspase form and a 30 and 10 kDa active form. The 17D9 antibody recognizes p43, p38 and p30 forms. Caspase-11 is an upstream protease that activates other caspases, such as caspase-3, -7 and -1, which in turn activate IL-1beta. Under Caspase-11-deficient conditions, cells are unable to process or secrete IL-1beta in response to stimuli and show defects in motility, which are due to disruption of its interaction with Aip1, an actin binding protein. Caspase-11 is homologous to human Caspase-4 (around 60%). Unlike other caspases which tend to be constitutively expressed, caspase-11 is upregulated by LPS treatment in macrophages, lymphocytes and hepatocytes.
Applications Reported: This 17D9 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 17D9 antibody has been tested by western blotting at 1-5 µg/mL on J774 cells or stimulated splenocyte lysates. Samples must be reduced for detection. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.