Immunofluorescence analysis of Caspase 3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 3 [3CSP03 (4.1.18)] Mouse Monoclonal Antibody (MA511521) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300).Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant full length human Caspase 3 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 6 publications below|
MA5-11521 targets Caspase 3 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human samples.
The MA5-11521 immunogen is recombinant full length human Caspase 3 protein.
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of caffeine on neuronal apoptosis in neonatal hypoxic-ischemic brain injury.
MA5-11521 was used in immunohistochemistry to study the effects of caffeine on hypoxia-ischemia-induced neuronal apoptosis in a neonatal rat model and the therapeutic implications for brain injury
|Kilicdag H,Daglioglu YK,Erdogan S,Zorludemir S||The journal of maternal-fetal and neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians (27:1470)||2014|
Combination of molecular alterations and smoking intensity predicts bladder cancer outcome: a report from the Los Angeles Cancer Surveillance Program.
MA5-11521 was used in immunohistochemistry to study the value of a panel of 9 biomarkers plus smoking intensity in predicting the outcome of bladder cancer
|Mitra AP,Castelao JE,Hawes D,Tsao-Wei DD,Jiang X,Shi SR,Datar RH,Skinner EC,Stein JP,Groshen S,Yu MC,Ross RK,Skinner DG,Cortessis VK,Cote RJ||Cancer (119:756)||2013|
Human papillomavirus in oral atrophic lichen planus lesions.
MA5-11521 was used in immunohistochemistry to study the involvement of human papillomavirus in oral atrophic lichen planus lesions
|Mattila R,Rautava J,Syrjänen S||Oral oncology (48:980)||2012|
Increased vein wall apoptosis in varicose vein disease is related to venous hypertension.
MA5-11521 was used in immunohistochemistry to study the effect of venous hypertension on vein wall apoptosis in varicose vein disease
|Filis K,Kavantzas N,Isopoulos T,Antonakis P,Sigalas P,Vavouranakis E,Sigala F||European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery (41:533)||2011|
Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis.
MA5-11521 was used in immunohistochemistry to study the immunohistochemistry of non-small cell lung cancer
|Yoo J,Jung JH,Lee MA,Seo KJ,Shim BY,Kim SH,Cho DG,Ahn MI,Kim CH,Cho KD,Kang SJ,Kim HK||Journal of Korean medical science (22:318)||2007|
Caspase-3 and caspase-6 in ductal breast carcinoma: a descriptive study.
MA5-11521 was used in immunohistochemistry to study caspase-3 and caspase-6 expression in ductal breast carcinoma.
|Blázquez S,Sirvent JJ,Olona M,Aguilar C,Pelegri A,Garcia JF,Palacios J||Histology and histopathology (21:1321)||2006|