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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant, full length human protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg of protein lysate|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25975 detects Caspase 3 from human samples.
MA1-25975 has been successfully used in immunoprecipitation, and Western blot procedures.
The MA1-25975 immunogen is recombinant, full length human protein.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; CPP-32; CPP32; CPP32B; cysteine protease CPP32; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1
CASP3; CPP32; CPP32B; SCA-1