|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rabbit, Rat, Insect, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Full-length recombinant human Caspase 3 protein|
|Contains||0.2% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for CASP3 (transient overexpression lysate), HEK 293 whole cell lysate.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Single-cell microinjection assay indicates that 7-hydroxycoumarin induces rapid activation of caspase-3 in A549 cancer cells.
MA1-16843 was used in western blot to test if 7-hydroxycoumarin induces changes in caspase-3 activity in human lung carcinoma cells
|Soto-Nuñez M,Díaz-Morales KA,Cuautle-Rodríguez P,Torres-Flores V,López-González JS,Mandoki-Weitzner JJ,Molina-Guarneros JA||Experimental and therapeutic medicine (10:1789)||2015|
Apoptosis in the pathogenesis of Nosema ceranae (Microsporidia: Nosematidae) in honey bees (Apis mellifera).
MA1-16843 was used in immunohistochemistry - paraffin section to study apoptosis in ventricular cells infected with N. ceranae
|Higes M,Juarranz Á,Dias-Almeida J,Lucena S,Botías C,Meana A,García-Palencia P,Martín-Hernández R||Environmental microbiology reports (5:530)||2013|
Exendin-4 improves hepatocyte injury by decreasing proliferation through blocking NGF/TrkA in diabetic mice.
MA1-16843 was used in immunohistochemistry to study the mechanism by which exendin-4 decreases proliferation and improves hepatocyte injury in diabetic mice
|Gezginci-Oktayoglu S,Sacan O,Yanardag R,Karatug A,Bolkent S||Peptides (32:223)||2011|
Antiapoptotic effect of angiotensin-II type-1 receptor blockade in renal tubular cells of hyperoxaluric rats.
MA1-16843 was used in immunohistochemistry to study the anti-apoptotic effect of an angiotensin-II type-1 receptor blocker in renal epithelial cells of hyperoxaluric rats
|Tunçdemir M,Demirkesen O,Oztürk M,Atukeren P,Gümü¿ta¿ MK,Turan T||Urological research (38:71)||2010|
Effect of vitamin C in reducing the toxicity of endosulfan in liver in rabbits.
MA1-16843 was used in immunohistochemistry to investigate the protective effect of vitamin C from endosulfan toxicity in liver
|Mor F,Ozmen O||Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (62:75)||2010|
Expression of caspase-3-dependent apoptosis in mural thrombi leukocytes.
MA1-16843 was used in immunohistochemistry to study caspase-3-dependent apoptosis in mural thrombi leukocytes
|Chu PH,Jung SM,Yeh CH,Wu HH,Shiu TF,She HC,Tseng NM||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (116:995)||2008|
|Not Applicable||Not Cited||
Decreased proliferation precedes growth factor changes after physeal irradiation.
MA1-16843 was used in immunohistochemistry to examine the earlier sequential relationship of proliferative, growth factor, and histomorphometric changes after irradiation leading to the 1-week nadir
|Damron TA,Horton JA,Naqvi A,Margulies B,Strauss J,Grant W,Farnum CE,Spadaro JA||Clinical orthopaedics and related research (null:233)||2004|
Effects of Z-FA.FMK on D-galactosamine/tumor necrosis factor-alpha-induced kidney injury and oxidative stress in mice : effects of Z-FA.FMK on TNF-alpha-mediated kidney injury.
MA1-16843 was used in western blot to examine the protective effect of cathepsin B inhibitor benzyloxicarbonyl-L-phenylalanyl-alanine-fluoromethylketone against kidney injuries
|Gezginci-Oktayoglu S,Tunali S,Yanardag R,Bolkent S||Molecular and cellular biochemistry (309:9)||2008|