|Tested species reactivity||Ferret, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||10-20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||10-20 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26426 detects active Caspase 3 from human, mouse, ferret, and rat samples.
PA1-26426 has been successfully used in immunohistochemistry (paraffin and frozen), Immunofluorescence, and Western blot procedures. Positive controls of rat brain or foetus, or mouse foetus suggested.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.