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Immunofluorescent analysis of Caspase-3 in A549 cells treated with staurosporine (right) and untreated (left) using a Caspase-3 recombinant rabbit monoclonal antibody (Product # 700182) at a dilution of 5 ug/ml followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000.
|Tested species reactivity||Human , Mouse|
|Published species reactivity||Porcine , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 171-175 of P42574.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||0.1-0.2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, feline, hamster, mouse, primate, pufferfish, rabbit, rat, porcine and Xenopus based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Caspases are a family of cysteine proteases that centrally controls apoptotic machinery. Caspases can be grouped according to their substrate specificities that are largely determined by the amino acids preceding the cleavage site. One group of caspases that include -6, -8, is specific for the substrate V/LEXD. This substrate is a site similar to those found in caspase proenzymes. This group of caspases may function as initiators of a proteolytic cascade by activating pro-caspases to amplify a death signal. A second group of caspases is specific for the substrate DEXD that is related to sites found on target proteins cleaved during apoptosis.
Caspase-3 is a member of the interleukin-1 beta converting enzyme, Caspase-8, nuclear lamins and others. The overexpression of Caspase-3 can result in apoptosis. Likewise, the inhibition of Caspase-3 or other caspases can prevent cells from entering the apoptotic pathway. Recent evidence has revealed a link between plasma caspase-3 and atherosclerosis and it role in activation of apoptosis in breast cancer mediated by siRNA-mediated Apollon silencing. This antibody is specific for the cleaved (active) form of caspase-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Investigating the role of shape on the biological impact of gold nanoparticles in vitro.
700182 was used in western blot to assess the impact of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells.
|Tian F,Clift MJ,Casey A,Del Pino P,Pelaz B,Conde J,Byrne HJ,Rothen-Rutishauser B,Estrada G,de la Fuente JM,Stoeger T||Nanomedicine (London, England) (10:2643)||2015|
Impact of diet deprivation and subsequent overallowance during gestation on mammary gland development and lactation performance.
||Farmer C,Palin MF,Martel-Kennes Y||Journal of animal science (92:141)||2014|
CASP-3, SCA-1, Apopain, mldy, A830040C14Rik, CPP-32, Lice, AC-3, CPP32, CPP32B, CC3, Caspase-3, Yama
SREBP cleavage activity 1, caspase 3, apoptosis related cysteine protease, caspase-3, cysteine protease CPP32, protein Yama, CASP-3, CPP-32, PARP cleavage protease, apopain, caspase 3, apoptosis-related cysteine protease, procaspase3, Yama, caspase 3, apoptosis-related cysteine protease