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      View (20) other Caspase 3 antibodies

      Invitrogen

      Caspase 3 Antibody (9H19L2), ABfinity™ Rabbit Monoclonal

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      Antibody target was verified by Cell Treatment to ensure the antibody binds to the antigen stated. View details.

      References Icon 10 References
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      Catalog #:700182

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      Product Details

      TESTED APPLICATIONS DILUTION
      Immunocytochemistry (ICC) 1-2 µg/ml
      Immunofluorescence (IF) 1-2 µg/ml
      Immunohistochemistry (Paraffin) (IHC (P)) 1:10-1:50
      Western Blot (WB) 0.1-0.2 ug/ml
      PUBLISHED APPLICATIONS
      Immunocytochemistry (ICC) See 3 publications below
      Western Blot (WB) See 3 publications below
      Immunohistochemistry (Paraffin) (IHC (P)) See 3 publications below
      Immunohistochemistry (IHC) See 1 publications below
      Tested Species reactivity Human, Mouse
      Published species reactivity Pig , Human
      Host / Isotype Rabbit / IgG
      Class Monoclonal
      Type Antibody
      Clone 9H19L2
      Immunogen A peptide corresponding to amino acids 171-175 of P42574.
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.5 mg/ml
      Purification Protein A
      Storage buffer PBS
      Contains 0.09% sodium azide
      Storage conditions Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
      RRID AB_2532293

      Product Specific Information

      This antibody is predicted to react with bovine, canine, feline, hamster, mouse, primate, pufferfish, rabbit, rat, porcine and Xenopus based on sequence homology.

      ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.

      ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.

      Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

      Background/Target Information

      Caspases are a family of cysteine proteases that centrally controls apoptotic machinery. Caspases can be grouped according to their substrate specificities that are largely determined by the amino acids preceding the cleavage site. One group of caspases that include -6, -8, is specific for the substrate V/LEXD. This substrate is a site similar to those found in caspase proenzymes. This group of caspases may function as initiators of a proteolytic cascade by activating pro-caspases to amplify a death signal. A second group of caspases is specific for the substrate DEXD that is related to sites found on target proteins cleaved during apoptosis.
      Caspase-3 is a member of the interleukin-1 beta converting enzyme, Caspase-8, nuclear lamins and others. The overexpression of Caspase-3 can result in apoptosis. Likewise, the inhibition of Caspase-3 or other caspases can prevent cells from entering the apoptotic pathway. Recent evidence has revealed a link between plasma caspase-3 and atherosclerosis and it role in activation of apoptosis in breast cancer mediated by siRNA-mediated Apollon silencing. This antibody is specific for the cleaved (active) form of caspase-3.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (10)

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      Bioinformatics

      Protein Aliases: Apopain; apoptosis-related cysteine protease; CASP-3; caspase 3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; Cysteine protease CPP32; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; Yama

      Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

      UniProt ID: (Human) P42574, (Mouse) P70677

      Entrez Gene ID: (Human) 836, (Mouse) 12367

      Molecular Function: cysteine protease enzyme modulator hydrolase protease protease inhibitor

      Function(s) Blue Arrow Icon
      response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance
      Process(es) Blue Arrow Icon
      protease binding
       aspartic-type endopeptidase activity
       cysteine-type endopeptidase activity
       cyclin-dependent protein serine/threonine kinase inhibitor activity
       death receptor binding
       protein binding
       peptidase activity
       cysteine-type endopeptidase activator activity involved in apoptotic process
       phospholipase A2 activator activity
       protein complex binding
       cysteine-type endopeptidase activity involved in apoptotic process
       cysteine-type endopeptidase activity involved in execution phase of apoptosis
       cysteine-type peptidase activity
       hydrolase activity

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