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Immunohistochemical analysis of Caspase 3 active & pro in formalin-fixed, paraffin-embedded human breast cancer using a Caspase 3 active & pro monoclonal antibody (Product # MA1-91637) at a dilution of 4 µg/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis. Cancer/normal adjacent tissue array was used for this test.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fusion protein (6xHis tagged full-length human Caspase 3 protein expressed in bacteria).|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||2-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91637 detects Caspase 3 active & pro from human samples.
MA1-91637 has been successfully used in Western blot and Immunohistochemistry(Paraffin) applications. Predicted molecular weight: 31kDa.
The MA1-91637 immunogen is recombinant fusion protein (6xHis tagged full-length human Caspase 3 protein expressed in bacteria).
Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; CPP-32; CPP32; CPP32B; cysteine protease CPP32; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1
CASP3; CPP32; CPP32B; SCA-1