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Immunofluorescence analysis of Caspase 6 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 6 (6CSP03 (14.1.190)) Mouse Monoclonal Antibody (MA511527) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human caspase 6 prodomain|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11527 targets Caspase 6 in IP and WB applications and shows reactivity with Human samples.
The MA5-11527 immunogen is recombinant human caspase 6 prodomain.
Caspases play a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis-inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase-6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Alternatively spliced caspase-6B isoform inhibits the activation of caspase-6A.
MA5-11527 was used in western blot to investigate the inhibitory effect of a caspase-6B isoform on caspase-6A
|Lee AW,Champagne N,Wang X,Su XD,Goodyer C,Leblanc AC||The Journal of biological chemistry (285:31974)||2010|
Self-activation of Caspase-6 in vitro and in vivo: Caspase-6 activation does not induce cell death in HEK293T cells.
MA5-11527 was used in western blot to investigate the effect of caspase-6 self-activation on cell death in HEK293T cells
|Klaiman G,Champagne N,LeBlanc AC||Biochimica et biophysica acta (1793:592)||2009|
Caspase-3 and caspase-6 in ductal breast carcinoma: a descriptive study.
MA5-11527 was used in immunohistochemistry to study caspase-3 and caspase-6 expression in ductal breast carcinoma.
|Blázquez S,Sirvent JJ,Olona M,Aguilar C,Pelegri A,Garcia JF,Palacios J||Histology and histopathology (21:1321)||2006|
apoptosis-related cysteine protease; apoptotic protease MCH-2; caspase 6; caspase 6, apoptosis-related cysteine peptidase; caspase 6, apoptosis-related cysteine protease; caspase-6