Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) stained using a Caspase 8 monoclonal antibody (Product # MA1-41280) at 4 ug/ml. Localization can be cytoplasmic and nuclear. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Caspase-8 amino acids PVETDSEEQP|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||4 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-41280 detects Caspase-8 in human, Rhesus monkey, and chimpanzee samples.
Suggested positive control: Jurkat, antigen standard for CASP8 (transient overexpression lysate), Jurkat whole cell lysate.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.