Search

  • Order Status
  • Quick Order
  • Promos

Invitrogen

Caspase 8 (Cleaved Asp384) Polyclonal Antibody

1 Reference
View all (2) Caspase 8 (Cleaved Asp384) antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Datasheet
Certificates
Protocols
Questions & Answers
Datasheet
Certificates
Protocols
Questions & Answers
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:

{{$ctrl.translations[$ctrl.isSkuNotesCollapsed ? 'antibody.pdp.skuNotes.viewMore' : 'antibody.pdp.skuNotes.viewLess']}}

Product Details

PA5-99435

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CASP8(Accession Q14790), corresponding to amino acid residues K367-P394.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818368

Product Specific Information

Antibody detects endogenous levels of fragment of activated Caspase 8 resulting from cleavage adjacent to Asp384.

Target Information

Caspase-8 (cleaved Asp384) refers to the activated form of caspase-8 generated following proteolytic cleavage at aspartic acid residue 384, a key step in the activation of this initiator caspase in the extrinsic apoptosis pathway. Caspase-8 is encoded by the CASP8 gene and is synthesized as an inactive zymogen (pro-caspase-8) containing tandem death effector domains (DEDs) that enable recruitment to death-inducing signaling complexes (DISCs) formed downstream of death receptors such as Fas (CD95), TRAIL receptors, and TNF receptor 1. Upon DISC assembly, pro-caspase-8 undergoes dimerization and autocatalytic cleavage at specific aspartic acid sites, including Asp384, producing active subunits that assemble into the functional enzyme. Activated caspase-8 initiates the apoptotic cascade by cleaving and activating downstream effector caspases such as caspase-3 and caspase-7, and can also process BID to link extrinsic and intrinsic apoptosis pathways. Detection of cleaved caspase-8 (Asp384) is widely used as a marker of death receptor-mediated apoptotic signaling in cell death and cancer research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: apoptotic cysteine protease; apoptotic protease Mch-5; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase precursor; Caspase-8 (Asp384 cleaved); caspase-8s; Cleaved Caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog

View more View less

Gene Aliases: ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5

View more View less

UniProt ID: (Human) Q14790

View more View less

Entrez Gene ID: (Human) 841

View more View less

Function(s)
endopeptidase activity cysteine-type endopeptidase activity death receptor binding protein binding peptidase activity cysteine-type peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process ubiquitin protein ligase binding death effector domain binding identical protein binding macromolecular complex binding scaffold protein binding
Process(es)
angiogenesis regulation of cytokine production regulation of immune system process proteolysis apoptotic process heart development extrinsic apoptotic signaling pathway via death domain receptors positive regulation of signal transduction regulation of tumor necrosis factor-mediated signaling pathway protein processing natural killer cell activation cell differentiation protein catabolic process macrophage differentiation positive regulation of cell migration response to cobalt ion response to estradiol response to lipopolysaccharide positive regulation of interleukin-1 beta production response to tumor necrosis factor TRAIL-activated apoptotic signaling pathway T cell activation B cell activation regulation of apoptotic process chordate embryonic development positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process regulation of innate immune response response to ethanol positive regulation of macrophage differentiation positive regulation of proteolysis animal organ development protein maturation negative regulation of necroptotic process syncytiotrophoblast cell differentiation involved in labyrinthine layer development pyroptosis cellular response to mechanical stimulus death-inducing signaling complex assembly response to anesthetic apoptotic signaling pathway extrinsic apoptotic signaling pathway execution phase of apoptosis self proteolysis positive regulation of execution phase of apoptosis
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support