|Tested species reactivity||Rat, Bovine, Sheep, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, corresponding to N terminal amino acids 1-134 of Human Caspase 9.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21141 detects Caspase 9 in human, mouse, rat, ovine, and bovine samples.
PA1-21141 has been successfully used in immunohistochemistry (paraffin) procedures. For IHC (p) staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, p.H. 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
PA1-21141 immunogen corresponds to synthetic peptide, corresponding to N terminal amino acids 1-134 of human Caspase 9.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.