Invitrogen

Caspase 9 (Cleaved Asp315) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

6 References

View all (2) Caspase 9 (Cleaved Asp315) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Caspase 9 (Cleaved Asp315) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Caspase 9 (Cleaved Asp315) was performed using 70% confluent log phase HeLa cells treated with 1µM of Staurosporine for 3 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 9 (Cleaved Asp315) Rabbit Polyclonal Antibody (Product # PA5-17913) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows untreated cells with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Caspase 9 (Cleaved Asp315) Antibody in Western Blot (WB) — Figure 9. miR-302d-3p regulates ERK signaling pathway by targeting transmembrane Bax inhibitor motif containing 6 to promote apoptosis of BC cells. (A) TUNEL assay was performed to detect the apoptosis rate of BC cells (scale bar, 100 um). Western blotting was performed to measure the expression of apoptosis-related proteins following transfection with (B) miR-302d-3p mimics or (C) miR-302d-3p inhibitor plus U0126 or EGF. *P<0.05, **P<0.01, ***P<0.001 vs. miR-302d-3p-NC; # P<0.05, ## P<0.01, ### P<0.001 vs. miR-302d-3p mimics or inhibitor. miR, microRNA; NC, negative control; ERK, extracellular signal-regulated kinase; BC, breast cancer.

Caspase 9 (Cleaved Asp315) Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Caspase 9 (Cleaved Asp315) using Anti-Caspase 9 (Cleaved Asp315) Rabbit polyclonal Antibody (Product # PA5-17913) shows expression of Caspase 9 (Cleaved Asp315) in HeLa cell line upon Staurosporine treatment.

Product Details

PA5-17913

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

1:100

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

6 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_10979038

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer's Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

PA5-17913 was used in Immunocytochemistry-immunoflourescence to establish a potential mechanism for the neurological defects observed in PNP-deficient patients and reinforce the critical role that PNP has for neuronal viability.

Tue May 31 00:00:00 EDT 2022

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

Tue May 31 00:00:00 EDT 2022

Stingray Venom Proteins: Mechanisms of Action Revealed Using a Novel Network Pharmacology Approach.

Marine drugs

Kirchhoff KN,Billion A,Voolstra CR,Kremb S,Wilke T,Vilcinskas A

PA5-17913 was used in Immunocytochemistry to develop a novel network pharmacology approach based on multi-omics functional data integration to predict how stingray venom disrupts the physiological systems of target animals.

Fri Dec 24 00:00:00 EST 2021

miR‑302d‑3p regulates the viability, migration and apoptosis of breast cancer cells through regulating the TMBIM6‑mediated ERK signaling pathway.

Molecular medicine reports

Liao Y,Qiu Z,Bai L

PA5-17913 was used in Western Blot to identify the role of miR‚302D‚3p in breast cancer and its potential mechanism.

Wed Dec 01 00:00:00 EST 2021

miR‑302d‑3p regulates the viability, migration and apoptosis of breast cancer cells through regulating the TMBIM6‑mediated ERK signaling pathway.

Molecular medicine reports

Liao Y,Qiu Z,Bai L

PA5-17913 was used in Western Blot to identify the role of miR‚302D‚3p in breast cancer and its potential mechanism.

Wed Dec 01 00:00:00 EST 2021

Inhibition of the prolyl isomerase Pin1 enhances the ability of sorafenib to induce cell death and inhibit tumor growth in hepatocellular carcinoma.

Oncotarget

Zheng M,Xu H,Liao XH,Chen CP,Zhang AL,Lu W,Wang L,Yang D,Wang J,Liu H,Zhou XZ,Lu KP

Published figure using Caspase 9 (Cleaved Asp315) polyclonal antibody (Product # PA5-17913) in Western Blot

Tue May 02 00:00:00 EDT 2017

Long Non-Coding RNA (lncRNA) Urothelial Carcinoma-Associated 1 (UCA1) Enhances Tamoxifen Resistance in Breast Cancer Cells via Inhibiting mTOR Signaling Pathway.

Medical science monitor : international medical journal of experimental and clinical research

Wu C,Luo J

PA517913 was used in immunocytochemistry to examine the role of UCA1 in tamoxifen resistance of estrogen receptor positive breast cancer cells

Fri Oct 21 00:00:00 EDT 2016

Human pluripotent stem cells have a novel mismatch repair-dependent damage response.

The Journal of biological chemistry

Lin B,Gupta D,Heinen CD

PA5-17913 was used in immunocytochemistry to explore DNA repair responses in human pluripotent stem cells

Fri Aug 29 00:00:00 EDT 2014

Human pluripotent stem cells have a novel mismatch repair-dependent damage response.

The Journal of biological chemistry

Lin B,Gupta D,Heinen CD

PA5-17913 was used in immunocytochemistry to explore DNA repair responses in human pluripotent stem cells

Fri Aug 29 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: APAF-3; apoptosis-related cysteine protease; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56

Gene Aliases: APAF-3; APAF3; CASP9; ICE-LAP6; MCH6; PPP1R56

UniProt ID: (Human) P55211

Entrez Gene ID: (Human) 842

Molecular Function: protease

Function(s)

proteolysis apoptotic process cellular response to DNA damage stimulus aging intrinsic apoptotic signaling pathway in response to DNA damage activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c platelet formation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process cellular response to UV signal transduction in response to DNA damage positive regulation of neuron apoptotic process response to antibiotic cellular response to dexamethasone stimulus extrinsic apoptotic signaling pathway in absence of ligand regulation of response to DNA damage stimulus

Process(es)

cysteine-type endopeptidase activity protein binding enzyme activator activity peptidase activity SH3 domain binding protein kinase binding cysteine-type endopeptidase activity involved in apoptotic process

Caspase 9 (Cleaved Asp315) Polyclonal Antibody

Product Details

PA5-17913

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!