Invitrogen

Cathepsin B Polyclonal Antibody

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Cathepsin B Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a CATB polyclonal antibody (Product # PA5-14255), followed by HRP-conjugated secondary antibody and DAB staining.

Cathepsin B Antibody in Western Blot (WB) — Western blot analysis using a CATB polyclonal antibody (Product # PA5-14255) in Y79 cell lysates (35 µg per lane).

Cathepsin B Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HepG2 cells using a CATB polyclonal antibody (Product # PA5-14255) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Product Details

PA5-14255

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-
Flow Cytometry (Flow)	1:10-1:50	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 198-227 amino acids from the central region of human CATB View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2245497

Product Specific Information

This antibody is predicted to react with non-human primate based on sequence homology.

Target Information

Cathepsin B is a lysosomal cysteine protease that has been implicated in the pathogenesis of rheumatoid arthritis, muscular dystrophy and tumor metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

L-leucyl-L-leucine methyl ester does not release cysteine cathepsins to the cytosol but inactivates them in transiently permeabilized lysosomes.

Journal of cell science

Repnik U,Borg Distefano M,Speth MT,Ng MYW,Progida C,Hoflack B,Gruenberg J,Griffiths G

PA5-14255 was used in Western Blotting to argue against the release of lysosomal enzymes into the cytosol and their proposed proteolytic signaling during LLOMe-induced apoptosis.

Fri Sep 15 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: amyloid precursor protein secretase; APP secretase; APPS; Cathepsin B; Cathepsin B heavy chain; Cathepsin B light chain; Cathepsin B1; cysteine protease

Gene Aliases: APPS; CPSB; CTSB

UniProt ID: (Human) P07858

Entrez Gene ID: (Human) 1508

Molecular Function: cysteine protease