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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli (His-Cathepsin D)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is SK-BR3 cells.
Cathepsin D (CatD) is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins apoptosis and autophagy. Human CatD is synthesized on the endoplasmic reticulum as a pre-pro-enzyme. Preprocathepsin D (412aa) is cleaved and glycosylated to form an inactive procathepsin D (392aa) and then further cleaved to generate an active intermediate (348aa) single-chain molecule. The active intermediate is further processed into mature two chain form of cathepsin D, this processing step is carried out by cathepsin B or L. The two-chain form consists of an amino terminal light chain and a carboxyl-terminal heavy chain. Additionally, several more amino acids are removed from the carboxyl terminus of the heavy chain. Procathepsin D (pCD), secreted from cancer cells, acts as a mitogen on both cancer and stromal cells and stimulates their proinvasive and pro-metastatic properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cathepsin D; ceroid-lipofuscinosis; ceroid-lipofuscinosis, neuronal 10; epididymis secretory sperm binding protein Li 130P; lysosomal aspartyl peptidase; lysosomal aspartyl protease; neuronal 10; OTTHUMP00000196039; OTTHUMP00000198692
CatD; CD; CLN10; CPSD; CTSD; HEL-S-130P