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|Host / Isotype||Human|
|Storage buffer||0.05M acetic acid, pH 5.5, with 0.25M NaCl|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RP-77525 is native Cathepsin G protein from human neutrophils. Purified from whole human blood shown to be non-reactive for HbsAG, anti-HCV, andit-HBc, and negative for anti-HIV1&2 by FDA approved tests.
RP-77525 may be used as a control with corresponding anti-Cathepsin G antibodies.
RP-77525 displays a specific activity of ~ 3000 mU/mg. One unit is defined as 1 umole of p-nitroanilide released per minute from 1 mM Suc-Ala-Ala-Pro-Phe-pNA in 100 mM Tris pH 7.5, 50 mM NaCL at 25 degrees C. The enzyme is about 90% active as tested by titration with alpha1-antichymotrypsin.
RP-77525 is inhibited by chymostatin, DFP and Z-Phe-CH2Br.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.