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FIGURE: 1 / 1
Cathepsin H Antibody (PA5-92424) in WB
Western blot analysis of Cathepsin H in extracts of various cell lines. Samples were incubated with Cathepsin H Polyclonal antibody (Product # PA5-92424) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-92424
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 23-140 of human CTSH (NP_0043812)
Conjugate
Form
Liquid
Concentration
1.6 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806491
Product Specific Information
Immunogen sequence: AELCVNSLEK FHFKSWMSKH RKTYSTEEYH HRLQTFASNW RKINAHNNGN HTFKMALNQF SDMSFAEIKH KYLWSEPQNC SATKSNYLRG TGPYPPSVDW RKKGNFVSPV KNQGACGS^M
^M
Positive Samples: K-562, A-549, HT-29; Cellular Location: Lysosome
Target Information
The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aleurain; Cat H; CATH; Cathepsin B3; Cathepsin BA; N-benzoylarginine-beta-naphthylamide hydrolase; Pro-cathepsin H
Gene Aliases: ACC-4; ACC-5; ACC4; ACC5; AL022844; CPSB; CTSH
UniProt ID: (Human) P09668, (Rat) P00786, (Mouse) P49935
Entrez Gene ID: (Human) 1512, (Rat) 25425, (Mouse) 13036
endopeptidase activity
aminopeptidase activity
cysteine-type endopeptidase activity
serine-type endopeptidase activity
protein binding
peptidase activity
cysteine-type peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
HLA-A specific activating MHC class I receptor activity
thyroid hormone binding
peptidase activator activity involved in apoptotic process
kininogen binding
protein complex binding
protein self-association
hydrolase activity
cysteine protease
metanephros development
T cell mediated cytotoxicity
adaptive immune response
immune response-regulating signaling pathway
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of epithelial cell migration
neuropeptide catabolic process
bradykinin catabolic process
positive regulation of peptidase activity
antigen processing and presentation
positive regulation of cell migration
zymogen activation
protein destabilization
response to retinoic acid
membrane protein proteolysis
negative regulation of apoptotic process
surfactant homeostasis
cellular protein metabolic process
positive regulation of angiogenesis
proteolysis involved in cellular protein catabolic process
dichotomous subdivision of terminal units involved in lung branching
ERK1 and ERK2 cascade
cellular response to thyroid hormone stimulus
positive regulation of apoptotic signaling pathway
spermatogenesis
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