Western blot of Cathepsin L in whole cell extract of Mv 1 Lu cultured mink lung using a Cathepsin L monoclonal antibody (Product # MA1-26774) at a dilution of 1:400 (Lane A) compared to a negative control with only secondary antibody (Lane B). The antibody was developed with a Goat anti-mouse IgG (Fab), AlkPhos Conjugate and NBT/BCIP substrate.
|Tested species reactivity||Human, Mustelid, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, full length, native protein from human lung cancer line EPLC 32 M1.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Gel Shift (GS)||See 1 publications below|
MA1-26774 detects Cathepsin L from human, mouse (weakly), rat, and mink samples.
MA1-26774 has been successfully used in immunohistochemistry (frozen), ELISA, and Western blot procedures.
The MA1-26774 immunogen is purified, full length, native protein from human lung cancer line EPLC 32 M1.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of an estrogen response element activated by metabolites of 17beta-estradiol and raloxifene.
MA1-26774 was used in EMSA assay to investigate the mechanism for the regulation of gene expression by 17 beta-estradiol and raloxifene
|Yang NN,Venugopalan M,Hardikar S,Glasebrook A||Science (New York, N.Y.) (273:1222)||1996|