Western blot analysis of Cathepsin L2 (Cleaved Leu114) in extracts from 293 cells and COS7 cells treated with etoposide (25µM, 1hour), and HeLa cells treated with etoposide (25µM, 24hrs) using a Cathepsin L2 (Cleaved Leu114) polyclonal antibody (Product #
|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from human CATL2|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cathepsin V (CTSU, CTSV, CATL2, cathepsin U, cathepsin L2) is a lysosomal cysteine proteinase that influences corneal physiology, and mediates degradation of invariant chain in human thymus. Cathepsin V is present in corneal epithelium, activated macrophages, and colorectal and breast carcinomas. A 1.8-kb mRNA to Cathepsin V is present in normal human thymus and testis. Cathepsin V is a member of the peptidase C1 family. Cysteine proteinases are synthesized as preproenzymes, which are processed to the corresponding proenzymes. The proenzymes are either targeted to the lysosome or continue along the cellular secretory route.
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