Immunofluorescent analysis of Caveolin 1 (green) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with a Caveolin 1 monoclonal antibody (Product # MA3-600) at a dilution of 1:100 for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Hamster, Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse|
|Immunogen||Intercellular membrane protein-containing vesicles (containing GLUT4) from rat.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||1 - 3 µl|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA3-600 detects caveolin from human, rat and hamster samples.
MA3-600 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a 19-21 kDa band from transfected HEK293 (B8) cells, with a non-specific band appearing at 65-70 kDa.
The MA3-600 immunogen is intercellular membrane protein-containing vesicles obtained from rat.
Caveolin-1 is through to play a part in the formations of caveolae, membrane trafficking, signal transduction pathways, and lipid homeostasis. Recently, a link between insulin signaling and caveolae has been suggested.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aquaporin-4 water channel oligomers are associated with the transverse tubules of skeletal myofibers.
MA3-600 was used in western blot to investigate the role of aquaporin-4 and its oligmers in the formation and function of T-tubules of skeletal myofibers
|Kaakinen M,Zelenin S,Metsikkö K||Experimental cell research (317:20)||2011|
Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.
MA3-600 was used in western blot to investigate the role of rat caveolae in lipid metabolism and trafficking in adipocytes.
|Souto RP,Vallega G,Wharton J,Vinten J,Tranum-Jensen J,Pilch PF||The Journal of biological chemistry (278:18321)||2003|