Invitrogen

Caveolin 1 Monoclonal Antibody (A3E4)

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Caveolin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Caveolin-1 in paraffin-embedded human lung carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with Caveolin-1 Monoclonal antibody (Product # MA5-45053) using a dilution of 1:1,000 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Caveolin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Caveolin-1 in paraffin-embedded human liver tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with Caveolin-1 Monoclonal antibody (Product # MA5-45053) using a dilution of 1:1,000 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Caveolin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Caveolin-1 in paraffin-embedded mouse lung tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with Caveolin-1 Monoclonal antibody (Product # MA5-45053) using a dilution of 1:1,000 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Caveolin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Caveolin-1 in paraffin-embedded human lung tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with Caveolin-1 Monoclonal antibody (Product # MA5-45053) using a dilution of 1:1,000 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Caveolin 1 Antibody in Western Blot (WB) — Western blot analysis of Caveolin-1 in SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in Caveolin-1 Monoclonal antibody (Product # MA5-45053) using a dilution of 1:500 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:5,000 for 1 hour at room temperature.

Caveolin 1 Antibody in Flow Cytometry (Flow) — Flow cytometry of Caveolin-1 in PANC-1 cells. The cells were fixed, permeabilized and stained with Caveolin-1 Monoclonal antibody (Product # MA5-45053) using a dilution of 1:50 (red) at room temperature for an hour followed by Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

MA5-45053

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:2,000	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	A3E4
Immunogen	Recombinant protein within human Caveolin-1 aa 1-150. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

Caveolin-1 or CAV1 is a scaffolding proteins within caveolar membranes. It interacts directly with G-protein alpha subunits and can functionally regulate their activity. Caveolin-1 is also involved in the co-stimulatory signal essential for T-cell receptor (TCR) mediated T-cell activation. It can bind with DPP4 and induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Mutations affecting the CAV1 gene can result in congenital generalized lipodystrophy 3, pulmonary hypertension primary 3, or partial lipodystrophy/congenital cataracts, and neurodegeneration syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: caveolae; caveolin 1, caveolae protein, 22kDa; caveolin, caveolae protein 1; caveolin, caveolae protein, 22 kDa; Caveolin-1; cell growth-inhibiting protein 32; HGNC:1527; OTTHUMP00000196475

Gene Aliases: BSCL3; CAV; Cav-1; CAV1; CGL3; LCCNS; MSTP085; PPH3; VIP21

UniProt ID: (Human) Q03135, (Mouse) P49817

Entrez Gene ID: (Human) 857, (Mouse) 12389

Molecular Function: scaffold/adaptor protein

negative regulation of transcription from RNA polymerase II promoter MAPK cascade inactivation of MAPK activity angiogenesis vasculogenesis response to hypoxia negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation negative regulation of cytokine-mediated signaling pathway response to ischemia regulation of the force of heart contraction by chemical signal triglyceride metabolic process calcium ion transport cellular calcium ion homeostasis regulation of smooth muscle contraction skeletal muscle tissue development response to nutrient lactation protein localization response to bacterium response to gamma radiation positive regulation of calcium ion transport into cytosol posttranscriptional regulation of gene expression positive regulation of gene expression negative regulation of muscle cell apoptotic process positive regulation of peptidase activity vesicle organization receptor-mediated endocytosis of virus by host cell regulation of fatty acid metabolic process lipid storage regulation of blood coagulation cholesterol transport negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of epithelial cell differentiation mammary gland development organ regeneration positive regulation of microtubule polymerization T cell costimulation negative regulation of protein ubiquitination positive regulation of protein ubiquitination receptor internalization negative regulation of protein binding positive regulation of protein binding maintenance of protein location in cell response to progesterone negative regulation of peptidyl-serine phosphorylation positive regulation of peptidyl-serine phosphorylation cholesterol efflux nitric oxide homeostasis positive regulation of toll-like receptor 3 signaling pathway wound healing vasoconstriction response to drug negative regulation of tyrosine phosphorylation of Stat5 protein cholesterol homeostasis positive regulation of catalytic activity negative regulation of MAPK cascade response to estrogen protein localization to plasma membrane raft negative regulation of nitric oxide biosynthetic process negative regulation of neuron differentiation positive regulation of endocytosis positive regulation of vasoconstriction negative regulation of JAK-STAT cascade microtubule polymerization negative regulation of pinocytosis negative regulation of smooth muscle cell proliferation leukocyte migration regulation of nitric-oxide synthase activity negative regulation of nitric-oxide synthase activity protein homooligomerization response to glucocorticoid regulation of cytosolic calcium ion concentration response to calcium ion membrane depolarization regulation of peptidase activity calcium ion homeostasis mammary gland involution positive regulation of cell adhesion molecule production negative regulation of necroptotic process negative regulation of protein tyrosine kinase activity negative regulation of ERK1 and ERK2 cascade caveola assembly cellular response to mechanical stimulus cellular response to exogenous dsRNA cellular response to peptide hormone stimulus cellular response to hyperoxia cellular response to transforming growth factor beta stimulus caveolin-mediated endocytosis regulation of heart rate by cardiac conduction angiotensin-activated signaling pathway involved in heart process negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway cellular senescence apoptotic signaling pathway regulation of membrane repolarization during action potential regulation of cardiac muscle cell action potential involved in regulation of contraction regulation of ventricular cardiac muscle cell action potential regulation of ruffle assembly negative regulation of peptidyl-tyrosine autophosphorylation negative regulation of potassium ion transmembrane transport regulation of cell communication by electrical coupling involved in cardiac conduction regulation of inward rectifier potassium channel activity positive regulation of ER-associated ubiquitin-dependent protein catabolic process protein localization to basolateral plasma membrane positive regulation of gap junction assembly beta-catenin destruction complex disassembly receptor internalization involved in canonical Wnt signaling pathway regulation of entry of bacterium into host cell negative regulation of anoikis positive regulation of extrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway negative regulation of cation channel activity regulation of the force of heart contraction endocytosis negative regulation of cell proliferation response to mechanical stimulus positive regulation of signal transduction negative regulation of signal transduction negative regulation of BMP signaling pathway negative regulation of MAP kinase activity protein oligomerization

Process(es)

RNA polymerase I transcription factor binding receptor binding patched binding structural molecule activity protein binding cholesterol binding peptidase activator activity enzyme binding protein kinase binding syntaxin binding protein binding, bridging protein complex scaffold identical protein binding ion channel binding protein heterodimerization activity Rac GTPase binding nitric-oxide synthase binding ATPase binding inward rectifier potassium channel inhibitor activity MDM2/MDM4 family protein binding SNARE binding kinase binding