Invitrogen

Caveolin 2 Monoclonal Antibody (ZC013)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

3 References

View all (17) Caveolin 2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Caveolin 2 Antibody in Western Blot (WB) — Western blot was performed using Caveolin 2 Monoclonal Antibody (ZC013) (Product # 41-0700) and a~18 kDa band corresponding to Caveolin-2 was observed in A431, A549, and HeLa; and was absent in Jurkat and 3T3L1. Whole cell extracts (30 µg lysate) of A431 (Lane 1), A549 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4) and 3T3L1 (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Caveolin 2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells tested owing to their inherent genetic constitution. Relative expression of TEAD1 was observed in A431, A549 and HeLa in comparison to Jurkat and 3T3L1 using Caveolin 2 Monoclonal Antibody (ZC013) (Product # 41-0700) in Western Blot.

Product Details

41-0700

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ZC013

Immunogen

Synthetic peptide derived from the C-terminal region of the human caveolin-2 protein

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533493

Target Information

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma.

Theranostics

Wang L,Yang C,Wang Q,Liu Q,Wang Y,Zhou J,Li Y,Tan Y,Kang C

41-0700 was used in Immunocytochemistry-immunoflourescence to study the role Cavin1-Caveolin1 interaction plays in regulating production and function of glioma-EVs.

Tue May 18 00:00:00 EDT 2021

Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma.

Theranostics

Wang L,Yang C,Wang Q,Liu Q,Wang Y,Zhou J,Li Y,Tan Y,Kang C

41-0700 was used in Immunocytochemistry-immunoflourescence to study the role Cavin1-Caveolin1 interaction plays in regulating production and function of glioma-EVs.

Tue May 18 00:00:00 EDT 2021

MicroRNA-218 inhibits cell migration and invasion in renal cell carcinoma through targeting caveolin-2 involved in focal adhesion pathway.

The Journal of urology

Yamasaki T,Seki N,Yoshino H,Itesako T,Hidaka H,Yamada Y,Tatarano S,Yonezawa T,Kinoshita T,Nakagawa M,Enokida H

41-0700 was used in western blot to study the role of miR-218 in renal cell carcinoma.

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: caveolae; caveolae protein, 20-kD; caveolin 2 isoform a and b; Caveolin-2; MGC12294; Phospho-CAV2

Gene Aliases: CAV; CAV2

UniProt ID: (Human) P51636

Entrez Gene ID: (Human) 858

Molecular Function: scaffold/adaptor protein

Function(s)

negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation vesicle fusion mitochondrion organization endoplasmic reticulum organization regulation of mitotic nuclear division insulin receptor signaling pathway vesicle organization receptor-mediated endocytosis of virus by host cell negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of MAPK cascade positive regulation of GTPase activity positive regulation by host of viral release from host cell positive regulation by host of viral process vesicle docking skeletal muscle fiber development protein oligomerization positive regulation of dopamine receptor signaling pathway caveola assembly

Process(es)

protein binding protein kinase binding protein binding, bridging protein complex scaffold protein homodimerization activity protein heterodimerization activity scaffold protein binding

Caveolin 2 Monoclonal Antibody (ZC013)

Product Details

41-0700

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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