|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment corresponding to the C-terminal residues 343-623 of human Cdc14A.|
|Storage buffer||PBS, pH 7.4, with 15mg/ml BSA|
|Contains||15mM sodium azide, 0.01% thimerosal|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-24747 detects Cdc14A from human samples.
MA1-24747 has been successfully used in immunoprecipitation, and Western blot procedures.
The MA1-24747 immunogen is a recombinant fragment corresponding to the C-terminal residues 343-623 of human Cdc14A.
The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human Cdc14A regulates Wee1 stability by counteracting CDK-mediated phosphorylation.
MA1-24747 was used in western blot to study the mechanism by which human Cdc14A regulates the stability of Wee1
|Ovejero S,Ayala P,Bueno A,Sacristán MP||Molecular biology of the cell (23:4515)||2012|