|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant CDC25A protein.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Mangrove dolabrane-type of diterpenes tagalsins suppresses tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest.
MA1-12293 was used in western blot to characterize suppression of tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest via mangrove dolabrane-type of diterpenes tagalsins
|Neumann J,Yang Y,Köhler R,Giaisi M,Witzens-Harig M,Liu D,Krammer PH,Lin W,Li-Weber M||International journal of cancer (137:2739)||2015|
The F box protein Fbx6 regulates Chk1 stability and cellular sensitivity to replication stress.
MA1-12293 was used in western blot to investigate the regulatory effect of Fbx6 on Chk1 turnover and on the cellular sensitivity to replication stress
|Zhang YW,Brognard J,Coughlin C,You Z,Dolled-Filhart M,Aslanian A,Manning G,Abraham RT,Hunter T||Molecular cell (35:442)||2009|
Dual regulation of Cdc25A by Chk1 and p53-ATF3 in DNA replication checkpoint control.
MA1-12293 was used in western blot to study the regulation of Cdc25A by Chk1 and p53-ATF3 in DNA replication checkpoint control
|Demidova AR,Aau MY,Zhuang L,Yu Q||The Journal of biological chemistry (284:4132)||2009|
The let-7 microRNA represses cell proliferation pathways in human cells.
MA1-12293 was used in western blot to study the role of let-7 microRNA in repressing human cell proliferation pathways
|Johnson CD,Esquela-Kerscher A,Stefani G,Byrom M,Kelnar K,Ovcharenko D,Wilson M,Wang X,Shelton J,Shingara J,Chin L,Brown D,Slack FJ||Cancer research (67:7713)||2007|
How adeno-associated virus Rep78 protein arrests cells completely in S phase.
MA1-12293 was used in western blot to determine how adeno-associated virus Rep78 protein arrests cells completely in S phase
|Berthet C,Raj K,Saudan P,Beard P||Proceedings of the National Academy of Sciences of the United States of America (102:13634)||2005|
Estrogen receptor beta inhibits 17beta-estradiol-stimulated proliferation of the breast cancer cell line T47D.
MA1-12293 was used in western blot to study the mechanism by which estrogen receptor beta inhibits 17beta-estradiol-stimulated proliferation of breast cancer cells
|Ström A,Hartman J,Foster JS,Kietz S,Wimalasena J,Gustafsson JA||Proceedings of the National Academy of Sciences of the United States of America (101:1566)||2004|
Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents.
MA1-12293 was used in western blot to investigate the role of Chk1 in the S and G2 arrests in response to DNA-damaging agents
|Xiao Z,Chen Z,Gunasekera AH,Sowin TJ,Rosenberg SH,Fesik S,Zhang H||The Journal of biological chemistry (278:21767)||2003|