|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||RBF/DnJ mice were immunized with recombinant human cdc25C fragment corresponding to amino acids 1-258 and fusing the splenocytes with NS-1 mouse myeloma cells. Epitope is known to be between amino acid residues 211-246.|
|Storage buffer||1mg/ml BSA, 50% glycerol|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||2.5 µg/ml|
|Immunoprecipitation (IP)||1 µg|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90555 detects CDC25c from human samples.
MA1-90555 has been successfully used in immunofluorescence, immunoprecipitation, Western blot applications.
The MA1-90555 immunogen is: RBF/DnJ mice were immunized with recombinant human cdc25C fragment corresponding to amino acids 1-258 and fusing the splenocytes with NS-1 mouse myeloma cells. Epitope is known to be between amino acid residues 211-246.
Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the
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