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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||RBF/DnJ mice were immunized with recombinant human cdc25C fragment corresponding to amino acids 1-258 and fusing the splenocytes with NS-1 mouse myeloma cells. Epitope is known to be between amino acid residues 211-246.|
|Storage buffer||1mg/ml BSA, 50% glycerol|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||2.5 µg/ml|
|Immunoprecipitation (IP)||1 µg|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90555 detects CDC25c from human samples.
MA1-90555 has been successfully used in immunofluorescence, immunoprecipitation, Western blot applications.
The MA1-90555 immunogen is: RBF/DnJ mice were immunized with recombinant human cdc25C fragment corresponding to amino acids 1-258 and fusing the splenocytes with NS-1 mouse myeloma cells. Epitope is known to be between amino acid residues 211-246.
Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cyclin dependent kinase 25c; dual specificity phosphatase CDC25C; mitosis inducer CDC25; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60
CDC25; CDC25C; PPP1R60