Western blot analysis of Cdc27 in (lane 1) HEK-293T (lane 2) MDBK (lane 3) COS7 (lane 4) NIH-3T3 (lane 5) P19 lysates. Samples were separated on SDS-PAGE and probed with a Cdc27 monoclonal antibody (Product # MA1-24749) at a dilution of 2 µg/ml. The antibody was developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat, Xenopus|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 814-823 of human Cdc27.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24749 detects Cdc27 from human, mouse, rat, Xenopus laevis, bovine, and canine samples.
MA1-24749 has been successfully used in immunohistochemistry (paraffin and frozen), ELISA and Western blot procedures.
The MA1-24749 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 814-823 of human Cdc27.
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.