|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full-length human Cdc34 expressed in E. coli.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||1-5 µg/ml|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23176 detects CDC34 in Human samples.
MA1-23176 has been successfully used in Immunoprecipitation and Western Blot procedures.
MA1-23176 immunogen corresponds to Full-length human Cdc34 expressed in E. coli..
Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.