This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details
MA3-029 detects Cdc37 from hamster, human, mouse and rat cells and tissues.
MA3-029 has been successfully used in Western blot, FACS, immunofluorescence, immunocytochemistry and immunoprecipitation (under denaturing conditions) procedures. By Western blot, this antibody recognizes a single 50 kDa protein representing Cdc37 in HeLa cell extract. Immunofluorescence staining of Cdc37 in Hepa1 cells with MA3-029 results in mostly cytoplasmic staining concentrated around the nuclear membrane. This antibody detects some weak non-specific bands in Jurkat, A431 and NIH-3T3 cell lysates.
The MA3-029 antigen is purified mouse Cdc37 fusion protein.
In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37.
It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck and Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDC37 (cell division cycle 37 S. cerevisiae homolog); CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; cell division cycle 37; cell division cycle 37 homolog; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co-chaperone Cdc37; Hsp90 co-chaperone Cdc37, N-terminally processed; p50Cdc37
Gene Aliases: CDC37; CDC37A; p50; P50CDC37