Invitrogen

Cdc37 Monoclonal Antibody (C1)

Cdc37 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

Cdc37 Antibody (MA3-029) in ICC/IF

Immunofluorescent analysis of Cdc37 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cdc37 monoclonal antibody (Product # MA3-029) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cdc37 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Cdc37 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cdc37 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Cdc37 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with Cdc37 siRNA and reduction of signal was observed in Western Blot using Anti-Cdc37 Monoclonal Antibody (Product # MA3-029). {KD}

Product Details

MA3-029

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:200

Flow Cytometry (Flow)

1:50

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Fruit fly, Human, Mouse, Rabbit, Yeast

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Purified mouse Cdc37 fusion protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2077740

Product Specific Information

MA3-029 detects Cdc37 from hamster, human, mouse and rat cells and tissues.

MA3-029 has been successfully used in Western blot, FACS, immunofluorescence, immunocytochemistry and immunoprecipitation (under denaturing conditions) procedures. By Western blot, this antibody recognizes a single 50 kDa protein representing Cdc37 in HeLa cell extract. Immunofluorescence staining of Cdc37 in Hepa1 cells with MA3-029 results in mostly cytoplasmic staining concentrated around the nuclear membrane. This antibody detects some weak non-specific bands in Jurkat, A431 and NIH-3T3 cell lysates.

The MA3-029 antigen is purified mouse Cdc37 fusion protein.

Target Information

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Alpha Isoform of Heat Shock Protein 90 and the Co-chaperones p23 and Cdc37 Promote Opioid Anti-nociception in the Brain.

Frontiers in molecular neuroscience

Lei W,Duron DI,Stine C,Mishra S,Blagg BSJ,Streicher JM

Published figure using Cdc37 monoclonal antibody (Product # MA3-029) in Immunohistochemistry

Fri Oct 20 00:00:00 UTC 2023

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.

Cell

Lopez VA,Park BC,Nowak D,Sreelatha A,Zembek P,Fernandez J,Servage KA,Gradowski M,Hennig J,Tomchick DR,Pawłowski K,Krzymowska M,Tagliabracci VS

MA3-029 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that phosphorylation of HSP90 prevents activation of immune receptors that trigger the hypersensitive response in plants.

Thu Sep 19 00:00:00 UTC 2019

PDLIM1 inhibits NF-κB-mediated inflammatory signaling by sequestering the p65 subunit of NF-κB in the cytoplasm.

Scientific reports

Ono R,Kaisho T,Tanaka T

MA3-029 was used in western blot to study how PDLIM1 suppresses NF-kappaB activation

Fri Dec 18 00:00:00 UTC 2015

PDLIM1 inhibits NF-κB-mediated inflammatory signaling by sequestering the p65 subunit of NF-κB in the cytoplasm.

Scientific reports

Ono R,Kaisho T,Tanaka T

MA3-029 was used in western blot to study how PDLIM1 suppresses NF-kappaB activation

Fri Dec 18 00:00:00 UTC 2015

Heat shock protein 90 controls HIV-1 reactivation from latency.

Proceedings of the National Academy of Sciences of the United States of America

Anderson I,Low JS,Weston S,Weinberger M,Zhyvoloup A,Labokha AA,Corazza G,Kitson RA,Moody CJ,Marcello A,Fassati A

MA3-029 was used in Western Blotting to demonstrate that heat shock protein 90 controls HIV-1 reactivation from latency by keeping the inhibitor of nuclear factor kappa-B kinase complex functional.

Tue Apr 15 00:00:00 UTC 2014

Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells.

Molecular biology of the cell

Pare JM,LaPointe P,Hobman TC

Published figure using Cdc37 monoclonal antibody (Product # MA3-029) in Western Blot

Thu Aug 01 00:00:00 UTC 2013

Functioning of the Hsp90 machine in chaperoning checkpoint kinase I (Chk1) and the progesterone receptor (PR).

Cell stress & chaperones

Felts SJ,Karnitz LM,Toft DO

Published figure using Cdc37 monoclonal antibody (Product # MA3-029) in Western Blot

Mon Mar 17 00:00:00 UTC 2008

Chaperoning checkpoint kinase 1 (Chk1), an Hsp90 client, with purified chaperones.

The Journal of biological chemistry

Arlander SJ,Felts SJ,Wagner JM,Stensgard B,Toft DO,Karnitz LM

Published figure using Cdc37 monoclonal antibody (Product # MA3-029) in Western Blot

Fri Feb 03 00:00:00 UTC 2006

Chaperoning checkpoint kinase 1 (Chk1), an Hsp90 client, with purified chaperones.

The Journal of biological chemistry

Arlander SJ,Felts SJ,Wagner JM,Stensgard B,Toft DO,Karnitz LM

Published figure using Cdc37 monoclonal antibody (Product # MA3-029) in Western Blot

Fri Feb 03 00:00:00 UTC 2006

Phase I trial of 17-allylamino-17-demethoxygeldanamycin in patients with advanced cancer.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Goetz MP,Toft D,Reid J,Ames M,Stensgard B,Safgren S,Adjei AA,Sloan J,Atherton P,Vasile V,Salazaar S,Adjei A,Croghan G,Erlichman C

MA3-029 was used in Western Blotting to characterise the maximum-tolerated dose, dose-limiting toxicities, efficacy, and pharmacokinetics of 17-allylamino-17-demethoxygeldanamycin in the treatment of advanced cancer.

Sun Feb 20 00:00:00 UTC 2005

p50(cdc37) is a nonexclusive Hsp90 cohort which participates intimately in Hsp90-mediated folding of immature kinase molecules.

Biochemistry

Hartson SD,Irwin AD,Shao J,Scroggins BT,Volk L,Huang W,Matts RL

MA3-029 was used in immunoprecipitation to investigate the role of p50(cdc37) in heat shock protein 90 molecular chaperone function

Tue Jun 27 00:00:00 UTC 2000

Cdc37 is a molecular chaperone with specific functions in signal transduction.

Genes & development

Kimura Y,Rutherford SL,Miyata Y,Yahara I,Freeman BC,Yue L,Morimoto RI,Lindquist S

MA3-029 was used in western blot to investigate the important roles of Cdc37 in signal transduction

Tue Jul 15 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: CDC 37; CDC37 (cell division cycle 37 S. cerevisiae homolog); CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; Cdc37 homolog; CDC37 protein; cell division cycle 37; cell division cycle 37 homolog; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co-chaperone Cdc37; p50Cdc37

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Gene Aliases: CDC37; CDC37A; p50; P50CDC37

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UniProt ID: (Human) Q16543, (Rat) Q63692, (Mouse) Q61081

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Entrez Gene ID: (Human) 11140, (Chinese hamster) 100760178, (Rat) 114562, (Mouse) 12539

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Function(s)

protein tyrosine kinase activity protein binding protein kinase regulator activity kinase binding protein kinase binding heat shock protein binding unfolded protein binding chaperone binding Hsp90 protein binding protein C-terminus binding mitogen-activated protein kinase kinase kinase binding protein kinase B binding chaperone

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity protein folding protein targeting posttranscriptional regulation of gene expression peptidyl-tyrosine phosphorylation ERBB2 signaling pathway protein stabilization regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway mitophagy in response to mitochondrial depolarization regulation of cell cycle regulation of protein kinase activity

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Cdc37 Monoclonal Antibody (C1)

Cdc37 Antibody (MA3-029) in ICC/IF

Product Details

MA3-029

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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