|Tested species reactivity||Human, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human Cdc6 protein and fusing the splenocytes with NS1/Ag4-1 mouse myeloma cells.|
|Storage buffer||1mg/ml BSA, 50% glycerol|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||1 µg|
|Western Blot (WB)||2.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91495 detects CDC6 from non-human primate, rat, human samples.
MA1-91495 has been successfully used in immunoprecipitation, Western blot applications.
The MA1-91495 immunogen is: Recombinant human Cdc6 protein and fusing the splenocytes with NS1/Ag4-1 mouse myeloma cells.
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.