|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Mice were immunized with recombinant human cdc6 protein and fusing the splenocytes with NS1/Ag4-1 mouse myeloma cells.|
|Storage buffer||1mg/ml BSA, 50% glycerol|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91494 detects CDC6 from non-human primate and human samples.
MA1-91494 has been successfully used in Western blot applications.
The MA1-91494 immunogen is recombinant human cdc6 protein and fused the splenocytes with NS1/Ag4-1 mouse myeloma cells.
The cdc6 protein, originally described in budding yeast (cdc6p), is essential and limiting for DNA synthesis. The protein functions as a replication initiation protein and as such is involved in the early steps of replication in eukaryotes serving as a "clamp-loader" for assembly of MCM proteins onto the replicating DNA. Recently the human homolog of the yeast cdc6 protein has been identified. The human cdc6 protein, p62cdc6, maps to chromosome 17q21.3 very close to the map position of the BRCA1 gene. Cdc6 is activated at the G1/S border by the activity of E2F3 and is expressed as a nuclear protein only in proliferating cells and not in quiescent cells. Recently cdc6 has been shown to interact specifically with the active cyclin A/cdk2 complex resulting in the phosphorylation of the cdc6 protein and a change in localization to the cytoplasmic compartment. Structurally, the cdc6 protein contains a cyclin dependent phosphorylation site, destruction boxes, a nucleotide binding/ATPase domain, and a potential leucine zipper suggesting an interaction with other proteins. Interestingly, the cdc6 protein has also been identified in a two-hybrid screen looking for PCNA interacting proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.